Gene Mapping Research Paper

Assignment of Gene Manipulation and Genetic Engineering

Review Article on topic: Significance of Gene Mapping in Molecular Diagnoses of Genetic diseases

Submitted to: Dr.Bashrat Hussain

Submitted by: Zuneira Saddique

Roll.No:

ms160200193

MS(Biotechnology)

Abstract; Genome mapping has great significance in molecular diagnosis of genetic diseases. It has opened new prospect of research and development in genetic studies and diagnosis of genetic diseases. Mapping also provides clues about which chromosome contains the gene and precisely where the gene lies on that chromosome. Genetic maps have been used successfully to find the gene responsible for relatively

When RFLPs were first described in 1980, a large effort was undertaken to generate maps of all the chromosomes. The first such maps were made in the early 1980s but covered only parts of chromosomes and had only a few markers. Maps of whole chromosomes were made by the late 1980s. By the mid-1990s, as the abilities of the research teams .After successful completion of human genome project, genetics of hereditary diseases are pulled the molecular biologist. Gene mapping is the most common method used to recognize a gene position in the genome. Restriction mapping can identify the allel type at specific position using restriction fragment length polymorphism(RFLP). Development of molecular techniques has helped to understand the molecular reasoning and mechanisms of genetic diseases.Gene mapping helps researchers to identify the locations on chromosomes at which specific gen exist indeed, linkage is critical for mapping and distinguishing genes, when we are trying to discover which gene is responsible for a specific phenotype. Genetic mapping also called as linkage mapping - can offer stable proof that a disease transmitted from parent to child is linked to one or more genes.In genetic screen mapping allows scientists to link genes to their

Huntingtin disease (HD)s is a rare, adult-onset, autosomal dominant,neurodegenerative disease. This disease was firstly identified by George Huntington in 1872. He explained the autosomal dominant inheritance pattern of this condition, which is accompanied by a loss of motor control leading to jerky movements, altered personality and psychiatric symptoms. HTT was first mapped to a specific chromosome in 1983. it was identified by a DNA probe that showed an HD-associated restriction fragment length polymorphism (RFLP) when DNA probe was used in Southern blot analyses of chromosomal DNA digested with the restriction enzyme HindIII (palindromic recognition sequence 5'-AAGCTT-3'). Then one probe called G8, showed a specific RFLP pattern which was linked to HD.Then by Using the G8 probe, the two HindIII sites known as H1 and H2 were palindromic within this chromosomal region. DNA fragments at these sites vary in length among different HD