Summary: Standard Curve For Glimepride

Figure No: 6.1- Standard Curve for Metformin Hcl

6.3.2. Standard Curve For Glimepride

Preparation of stock solution An accurately weighed quantity of 50mg of Glimepride was taken in a clean, dry 100ml of volumetric flask. Then volume was made upto 100ml with Methanol and the solution was mixed properly by sonication for 30mins. From that 2ml was drawn and transfer into a 100ml volumetric flask and volume was made upto 100ml.

Determination of λmax

The absorbance of the solution was scanned from 200-400nm wavelength in UV-Visible Spectrophotometer(Model no- V-630). In the present work the maximum absorbance (λmax) of Glimepride was found at 228nm.

Preparation of standard dilution From the stock solution, 1ml, 2ml, 3ml, 4ml, 5ml solution

MANUFACTURING PROCEDURE (General)

Step: 1 – All ingredients were weighed in specified quantity as given in the formula.

Step: 2 –Metformin Hydrochloride and Polymer (DCP, HPMC K100M, HPMC K4M, HPMC K15M,) PVP-K30, MCC (Avicel ph-102), Gelatin was sifted through 40# sieve.

Step: 4 - The step 2 ingredients(except gelatin) were loaded into planetary mixer and mixed for 30 minutes.

Step: 5 – Talc, and Magnesium Stearate was sifted through 40# sieve.

Step: 6 –Then a paste was prepared by using 100 ml of DM hot waterand gelatin +(a solution of starch in water previously prepared but in case of IPA granulation where starch and gelatin are not used IPA was added to the step 4 mixture.).

Step: 7- Then to the step 4 shifted mixture the paste was added slowly with water (q.s) and mix up in a Rapid Mixture Granulator.

Step: 7 – Finally when granules are seemed to be formed they are transferred to tray dryer for sufficient drying and then the granules were again passed through 16# sieve. Then accurately weighed Glimepride was added to the prepared granules at lubrication time by Geometric Mixing method, and then the granules were compressed into tablets and evaluated for all physical and chemical