Genetically Modified Organisms: A Case Study

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The amount of Genetically Modified Organisms and the number of varieties of the Genetically Modified Organisms (GMO), keep increasing continuously as the science leads towards for the betterment of the humankind and nature. The quantification of the GMO become an ultimate necessity as the usage and environmental release of the Genetically Modified Organisms has become a great concern of the society. The social concern on the GMO fall into three major types, a portion of society admirers the advances of genetics and support the advancement and another portion appears to counteract on it,1 and is willing to pay more to avoid GM food.2 while the remainder is unaware or neutral on GMO. A GMO is an organism which the genetic material or the genome…show more content…
It is done in a way that doesn’t occur by any natural process but by application of molecular biological and biochemical knowledge and technology. Currently, the GM plants are in the most global importance. The correct information should be provided on the Genetically Modified Organisms content during the marketing of the GMO and GM products. Therefore, the labelling and informative requirements have been set by certain governments and certain authorities in order to facilitate the global cultivation and international trading of the GMO and GM products.…show more content…
In order to achieve a better comparison, an additional sample is assayed by using different quantification methods. Different varieties of the GMOs are available and more mutations may occur in the inserted sequence during crossing. The selection of the taxon-specific target, requires extensive knowledge on the genetic structure of the taxon and closely related taxon, as it should differentiate the interested taxon from related taxon, and also fulfil certain other requirements. As different measurement units are being used by different laboratories, harmonization of methods is required. These are some of major challenges in the qPCR approach. As the amount of different GMOs are rapidly progressing, the necessity to look for new strategies arises. As a result, new statistical strategies, development of the digital PCR and next generation sequencing (NGS) for the GMO quantification purposes appears to become best solutions and also a challenge for the future. DNA microarray, multiplex PCR methods,5 and capillary gel electrophoresis provide some solutions for some problems. The DNA microarray enables the detection of multiple targets in a single platform. The multiplex PCR expands

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