ipl-logo

Glucouronidase Test Essay

916 Words4 Pages

Following are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Beta glucouronidase test is used for the identification of Escherichia coli. An enzyme is produced by E.coli which is beta D glucouronidase. Beta d glucouronidase in turn hydrolyzes beta d glucopyranosid uronic derivatives to aglycons and D glucuronic acid. Bile solubility test is used in laboratory for differentiation of alpha hemolytic Streptococci from Streptococcus pneumoniae.
In catalase test it acts as a catalyst for the breakdown of hydrogen peroxide to oxygen and water. Catalase production test is done for an organism by bringing it into association with hydrogen peroxide. If an organism is …show more content…

In the test for a reducing sugar, if it changes to a red orange color is it known as a precipitate as it comes out of the solution and forms solid particles dispersed around the water. The test of non-reducing sugar is striving as a result of if there is any sucrose presents it is broken down into those monosaccharides, which can be proved for using the common reducing sugar test. A positive result indicates that non-reducing sugars are present on the original sample. Sucrose fermentation it involves inoculating of sucrose broth with inoculating loop. Usually done for the differentiation of Enterobacteriaceae species. A positive result gives yellow color after the addition of indicator. A negative result shows no change in color or has red color. In glucose fermentation inoculation of broth is done by using inoculating loop. This test distinguishes Enterobacteriaceae family. This test is done for testing bacteria ability to ferment glucose and gas production. Yellow color shows the positive result for an acid. For gas positive result bubbles are seen in the test tube. A negative result shows either reddish color or no color change and also no gas

Show More
Related

  • Unknown Culture Lab Report

    1054 Words  | 5 Pages

    Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.

    Read More

  • Lab Report Identifying Unknown Microorganisms

    1596 Words  | 7 Pages

    I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided

    Read More

  • Unknown Bacteria Number 5 Lab Report

    1114 Words  | 5 Pages

    This test was conducted utilizing aseptic technique. I first properly labeled the test tube and aseptically inoculated an MR-VP broth tube with unknown bacteria number 5 using a sterile inoculating loop. After 4 days of incubation, I added 4 drops of methyl red ph indicator to the tube. The contents of the test tube turned yellow in color. This indicates a negative test result because unknown bacteria 5 utilized the butanediol pathway instead of the mixed acid fermentation pathway.

    Read More

  • Ca-Mrsa Case Studies

    1264 Words  | 6 Pages

    In lab, we learned to distinguish two types of bacteria using the gram stain, gram positive and gram negative. Since S. Aureus has a thick peptidoglycan cell wall with teichoic acid, it stains gram positive. Further tests will indicate the bacteria, including the presence of catalase, sheep blood hemolysis, mannitol fermentation, halotolerance, and coagulase, which S. Aureus is positive for all. 3.)

    Read More

  • Unknown Microbiology Essay

    517 Words  | 3 Pages

    Catalase activity test establishes whether the bacterium produces the enzyme catalase. The eosin methylene blue test or EMB, inhibits the growth of gram positive bacteria and tests whether or not gram negative bacteria can ferment lactose. Lactose fermentation testing is done to see if the bacterium is capable of fermenting sugar by testing for acid and gas production. These are the possible tests that are needed in order to identify unknown

    Read More

  • Respiration And Crayfish

    146 Words  | 1 Pages

    While CO2 is produced, the amount of CO2 produced is different depending on the organisms, in this case crayfish. To test the changes in pH, NaOH is used to neutralize the carbonic acid produced by the crayfish, by which the

    Read More

  • Serum 2216 Lab Report

    592 Words  | 3 Pages

    The investigation was carried out to identify the presence or absence of biological molecules in serum 2216. If the concentration in each test tube of the dilutions carried out will be more concentrated then the concentration of the test tube before it, then the color will be at an equal concentration with the other dilutions performed. The hypothesis was wrong because of the difference in concentrations due to the different measurements within the dilutions done. The test for starch was to add a drop of iodine solution to the pipette in the spotting tile. A reducing sugar solutions is add inside a test tube with 3 drops to then add 3 drops of benedicts and plane in a water bath.

    Read More

  • Lab Report On Enzyme Activity

    1419 Words  | 6 Pages

    Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.

    Read More

  • Macromolecules Lab Report

    1486 Words  | 6 Pages

    The starch-iodide complex forms because of the transfer of charge between the starch and iodide ion and results in spacing between the energy levels. This allows the complex to absorb light at different wavelengths resulting in a dark blue colour (Travers et al., 2002). A blue colour would indicate a positive test while a yellow colour would show a negative test. The Benedict’s test is useful for reducing sugars.

    Read More

  • Exercise 14 Unknown Identification Lab Report

    449 Words  | 2 Pages

    Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.

    Read More

  • Laboratory Report on Enzyme Activity

    1407 Words  | 6 Pages

    55 degrees celcius Table 6: Effect of Sucrose Concentration on Sucrase Activity Optical Density 35 g/L 30 g/L 25 g/L 20 g/L 15 g/L 10 g/L 5 g/L 0 g/L 1 1.007 0.974 0.950 0.926 0.849 0.734 0.515 0.003 2 1.002 1.011 0.947 0.937 0.834 0.766 0.496 0.002 3 0.980 0.998 0.944 0.932 0.838 0.754 0.495 0.001 average 0.996 0.994 0.947 0.932 0.840 0.751 0.502 0.002 Effect of Sucrose Concentration on Sucrase Activity 5. State how sucrase activity changes with increasing sucrose concentration. First sucrase activity increases greatly. After 10 g/l sucrase activity continues to increase but at a slow rate until it reaches 30 g/l. At 30 g/l to 35 g/l sucrase activities mostly stayed the same

    Read More

  • Hydrothermal Vents Essay

    629 Words  | 3 Pages

    The tests performed to determine these characteristics are: a Feulgen Stain test to determine if the sample contains DNA or not, a Fat test to determine the absence or presence of fats and oils, an Iodine test to determine the absence or presence of starch, a Biuret test to determine whether the sample contains protein or not, a Benedict’s test to determine whether the sample contains a low, high or no amount of reducing sugars, and finally a Tetrazolium test to determine whether the sample is alive or

    Read More

  • Catalase Test

    920 Words  | 4 Pages

    Pyruvate can be metabolized into neutral compounds such as acetoin, 2-3 butylene glycol, and diacetyl. [2 pyruvate -> acetoin + 2CO2 ; acetoin + NADH + H+ -> 2,3-butanediol + NAD+] “In the presence of alkali (KOH) and atmospheric oxygen, acetyl methyl carbinol is oxidized to diacetyl, a reaction which is catalyzed by alpha- naphthol. Diacetyl formed reacts with guanidine-containing compounds such as arginine contributed by peptone in the medium, to form a red colored product. The resultant red color is indicative of a positive VP test.”

    Read More

  • Lab Report On Fermentation

    1432 Words  | 6 Pages

    Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.

    Read More

  • Essay On Microorganism

    1328 Words  | 6 Pages

    There are some kinds of characteristic such as coccus, bacillus, and spiral. The example of coccus could be such as Staphylococcus aureus, streptococcus and bacteria types-Gram stained. We can observe that these bacteria have purple colour because these bacteria having peptidoglycan to their cell wall. In Gram staining, the outer lipid-based membrane of gram-negative bacteria is removed by an alcohol solution. The alcohol also decolorizes the exposed peptidoglycan layer by dissolving away the previously applied crystal violet.

    Read More

More about Glucouronidase Test Essay

Open Document