After a gram stain was done unknown #257 was identified as a gram positive organism because when observed under the microscope the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with a heavy quadrant one.
In the Oxidative fermentation tube the media was a differential media that helps determine whether specific bacteria can oxidize or ferment to metabolize glucose. Citrate test checks to see which bacteria could citrate as the only source of carbon. A positive test shows that an alkaline environment ia created and that the pH level rose. The color of the media changed from green to blue if its positive. The Bile Esculin agar test has its medium as selective and differential.
Compare the result to the chart on the back of the urinary pH test strips bottle, and record data. Clean the stirring rod with water before moving on to the next test tube. Repeat this process for each increment (2 mL, 3mL, 4mL) Figure #1: Picture of bean solution mixed Figure #2: Picture of materials needed for the with alpha galactosidase experiment Safety considerations: Be careful with the beakers, glass stirring rod, and test tubes, as they could break easily and can cause cuts in the skin. DCP: A scatter plot will be used to display how the amount of alpha galactosidase (measured in mL) in the bean solution affects the glucose concentration (measured in mg/dL) and error bars to show the standard deviation. A line of best fit will be used to show the relationship between the glucose concentration and the amount of alpha galactosidase.
The reaction that occurs can be investigated via the adding of the liver extract which contains the arginase to urea concentrations and distilled water. The amount of urea formed is determined via spectrophotometric analysis α-INPP. The urea produced was known via the color reaction with the α-INPP, it is the reagent used for the colorimetric determination of urea. (Barry J, et al. 1984).
Inoculate the test agar medium: There are two types of inoculation that can be done. Phenol Red Broth – Glucose, Lactose, Sucrose The test results are as follows: for glucose, lactose, and Unknown 361 tested A/-, meaning that acid was produced, but no gas and it tested K for sucrose meaning that there was alkaline production. Procedure: 1. Obtain one tube for each sugar, usually one for glucose, lactose, sucrose, mannitol. Do not get them confused, they look the same, it is suggested that they be labelled immediately if they are not already labelled.
It was found that the compound was solid and white in color. The unknown compound was then tested solubility in water and the compound was soluble in the water. The flame test was performed for four know compound calcium chorine, sodium chlorine and ammonium chorine and the unknown compound. When unknown compound was put on the fire different color are produce. When we smell the unknown compound it indicated the presence of chorine.
Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome. Lastly, a glucose fermentation test was conducted to determine the unknown organism.
Therefore, acid fat, lactose fermentation, mannitol fermentation were not needed to be performed, because they are selective to a specific thing. As a result, Unknown bacteria “W” was concluded to be gram stain positive, endospore positive, bacilli shape (rod shape), and arranged in chains (strepto-). Test Purpose Reagents Observation Results Gram Stain To determine gram reaction of bacteria. Crystal violet, Grams iodine, Alcohol, and Safranin Purple streptobacilli bacteria was observed. Gram positive rods.
The purpose of this experiment is to perform a two step reductive amination using o-vanillin with p-toluidine to synthesize an imine derivative. In this experiment, 0.386 g of o-vanillin and 0.276 g of p-toluidine were mixed into an Erlenmeyer flask. The o-vanillin turned from a green powder to orange layer as it mixed with p-toludine, which was originally a white solid. Ethanol was added as a solvent for this reaction. Sodium borohydride was added in slow portion as the reducing agent, dissolving the precipitate into a yellowish lime solution.
The result of the reaction was then recorded in notes. The positive control was the color that changes after the protein, and the negative control was the sample without treatment. The dependent variable is the color change. The independent variable is the chemical reaction with the reagent. The treatment group is Supplement #1, 3, and 9.