Sodium Gluoride Lab Report

“BEST GLYCOLYTIC INHIBITOR – D,L GLYCERALHYDE VS SODIUM FLUORIDE FOR ESTIMATION OF GLUCOSE”

Author: DR.G.UDAYA KUMARI, PROF.V.MEERA,

Department of Biochemistry, Kilpauk Medical College and hospital, Chennai.

ABSTRACT:

AIMS: To determine an ideal antiglycolytic agent - D,L Glyceraldehyde (GLY) or Sodium Fluoride(NaF) for estimation of glucose..MATERIALS AND METHODS: 80 random blood samples were collected from the OP patient after obtaining explicit consent. 6 ml of venous blood was extracted from each patient and was equally distributed between GLY tube, NaF tube and Plain tube with clot activator(PTC).GLY tube was prepared at the concentration of 5mmol/L.Serum from the PTC were separated after 30 minutes and refrigerated at 4°C until analysed. Serum from

Coated tubes were allowed to dry for a period of 48 hours at 37°C. After complete drying, tube cap was applied. Final concentration of GLY was 5mmol/l.. NaF tubes and PTC tubes of 2ml capacity were procured. 6 ml of venous blood was extracted from each patient and it was equally distributed - 2ml in PTC tube (5 inversions), 2ml in NaF tube (8 inversions) and 2ml in the tubes coated with 5mmol/L of GLY (8 inversions). Serum from the PTC were separated after 30 minutes and refrigerated at 4°C until analysed. Serum from the NaF and GLY coated tubes were put at 25°C for a period of 8hrs and were centrifuged for 10 minutes ,at 3000 rpm .serum separated from the above samples was used for the estimation of glucose by GOD-POD method in Merck microlab 300 semiauto analyser.

QUALITY CONTROL

Serum Glucose analyses were done using RANDOX calibrator (lot no 2351-562UE) and 2 level of Controls (lot no 768UN and lot no 501UE ) used for checking internal quality.

ESTIMATION OF SERUM GLUCOSE

Method: Glucose oxidase peroxidase (GOD/POD)

Kit Used: Erba

Principle

Glucose