Glycolysis Lab Report

Glycolysis

Introduction

• Definition :

The process in which two molecules of pyruvate are formed by the metabolism of glucose through series of enzymatic reaction.

• Purpose :

Glycolysis is involved in the production of adenosine triphosphate (atp) which provide energy to the body [1].

Glycolysis is anaerobic process which is carried out in the cytosol of the cells. Its metabolic steps occur in the absence of oxygen. Because living things first appeared in the environment lacking oxygen , so it is believed that glycolysis was an early and important metabolic pathway involved in extracting energy from the metabolism of the food. It also play an important role in the aerobic process during the biological evolution of the earth. Organism still use

Transfer of phosphate from 1,3-dpg to adp

This is the first step of glycolysis involve in the generation of atp. In this reaction carboxylic group of 3-phosphoglyceraldehyde transfer high-energy phosphate group to adp by the help of enzyme phosphoglycerate kinase. As a result of this atp is generated [6].

8. Isomerization of 3-phosphoglycerate

This is reversible reaction which involves the intramolecular shifting of phosphoryl group from c-3 to c-2 resulting in the conversion of 3- phosphoglycerate into 2-phosphoglycerate. This reaction is catalyzed by phosphoglycerate mutase [6].

9. Dehydration of 2- phosphoglycerate

In this reaction dehydration of 2-phosphoglycerate occur in the presence of enolase ( phosphopyruvate hydratase ) to phosphoenolpyruvate. This is reversible reaction in which phosphoenolpyruvate conatain super high energy phosphate bond [6].

10. Transfer of phosphate from pep to adp

This is second atp generating step in glycolysis. In the presence of allosteric enzyme pyruvate kinase phosphoenolpyruvate is converted into pyruvate in enol form. This enzyme is involve in the transfer of phosphoryl group from phosphoenolpyruvate to adp resulting in the generation of atp [6].

Preparatory phase