Glyphosate Herbicide Lab Report

BIODEGRADATION OF PESTICIDE GLYPHOSATE USING FUNGUS.

ABSTRACT

Glyphosate [N-(phosphonomethyl) glycine] is a broad- spectrum systemic herbicide. Because of the ever-increasing application of glyphosate in agricultural fields and detection of its presence in soils and environmental waters, there is a growing concern that it may be potentially harmful to humans and animals. A fungal strain was isolated from the soil sample around VIT through enrichment with M1 medium and designated as WAM. Firstly, MIC (Minimum inhibitory concentration) of the herbicide was carried out to determine the tolerance of the herbicide and was found to be 500µg/l. Subsequently with the same pesticide concentration the degradation of the herbicide by the fungus was done. The samples collected at regular intervals were tested with HPLC, FTIR, GC-MS. Growth kinetics of the organism was conducted in M1 medium. The fungal strain was further investigated with18s rRNA sequencing for identification.

1, INTRODUCTION

Herbicides (weed killers) are pesticides used to kill unwanted plant materials. Herbicides are generally very selective in their toxicity. They leave the desired crop relatively unharmed. The potential effects of herbicides are strongly influenced by their toxic mode of action and their method of application. Herbicides may cause biological impairments of

Firstly, fungal culture was inoculated from pure culture into potato dextrose broth (PDB) at 35°c for 7 days. 1 ml of this fungal suspension was then transferred to 7 different flasks containing 100 ml M1 broth with increasing concentration of glyphosate i.e., from 25µl in 100mg to 171µl in 700mg.The flasks were then incubated on shaker at room temperature for 7 days. After incubation, the content of flasks were filtered using filter paper, the residue was collected and weighed. The dry weights of filter papers were taken