Glyphosate Research Paper

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BIODEGRADATION OF PESTICIDE GLYPHOSATE USING FUNGUS.

ABSTRACT
Glyphosate [N-(phosphonomethyl) glycine] is a broad- spectrum systemic herbicide. Because of the ever-increasing application of glyphosate in agricultural fields and detection of its presence in soils and environmental waters, there is a growing concern that it may be potentially harmful to humans and animals. A fungal strain was isolated from the soil sample around VIT through enrichment with M1 medium and designated as WAM. Firstly, MIC (Minimum inhibitory concentration) of the herbicide was carried out to determine the tolerance of the herbicide and was found to be 500µg/l. Subsequently with the same pesticide concentration the degradation of the herbicide by the fungus was
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Herbicides are generally very selective in their toxicity. They leave the desired crop relatively unharmed. The potential effects of herbicides are strongly influenced by their toxic mode of action and their method of application. Herbicides may cause biological impairments of water bodies if they occur in water or sediment at sufficient concentrations. Most commonly they enter surface runoff or leachate, but because they have relatively low toxicity to fish and invertebrates, acute toxicity is likely only when they are deliberately or accidently applied directly to water bodies. Some herbicides cause a range of health effects ranging from skin rashes to death. Phenoxy herbicides are often contaminated with dioxins such as TCDD which can cause cancer. Commercial herbicide use generally has negative impacts in bird…show more content…
It’s mode of action as an herbicide is to inhibit a plant enzyme involved in the synthesis of aromatic amino acids: tyrosine, tryptophan and phenylalanine. It is absorbed through foliage, minimally through roots, and translocated to growing points. Due to this limitation it is effective only in actively growing plants. Glyphosate adsorbs strongly to soil, and residues are expected to generally be immobile in soil. Ground and surface water pollution is limited. It is readily degraded by soil microbes to aminomethylphosphonic acid (AMPA). Half-life of glyphosate in soil ranges between 2 and 197 days, a typical field half-life of 47 days.

2. MATERIALS & METHODOLOGY

2.1 SAMPLE COLLECTION
Soil sample was collected from different region around VIT premises. The soil sample collected was taken for serial dilution.
1 gram of the soil was taken and then added to a 10 ml distilled water in a test tube. The sample was subjected to a 10 fold serial dilution. 0.1 ml sample was taken from 10-3 and 10-4 dilution and inoculated on a potato dextrose agar (PDA) plates using spread plate method. The plates were then incubated at 35°c for 7 days.
2.2 ISOLATION & ENRICHMENT
After incubation, a colony from each isolates were taken and inoculate on Czapek dox agar plates using spot inoculation technique for enriched

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