Gooseberry Lab Report

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APPLICATION OF RESPONSE SURFACE METHODOLOGY FOR THE OPTIMISATION OF Cr(VI) BIOSORPTION USING GOOSEBERRY SEED J Aravind*, P Kanmani, G Sudha, R Balan Department of Biotechnology, Kumaraguru College of Technology, Chinnavedampatti, Saravanampatty, Coimbatore, Tamilnadu – 641 049 *corresponding author Email ID : dr.j.aravind@gmail.com Telephone : (+91)9443518878 Fax No. :0422-2669406 ABSTRACT The current investigation portrays the role of Gooseberry (Phyllanthusacidus) seed as an effective biosorbent for remediating Chromium (Cr (VI)), a toxic heavy metal pollutant common in effluents from tanneries and its allied industries. Biosorption is affected byfactors like pH, temperature and initial metal concentration. Further there …show more content…

The seeds were washed under running tap water to remove the dirt and other particulates. It was subjected to drying at 40C for 24 h. Then the seeds were finely ground and sieved. The finely powdered biosorbent was subjected to washing. After washing with distilled water, the mixture was filtered and dried at 50C for a period of 1 h. The dried powder was stored in an air tight container to prevent moisture. The dried powder was used as a biosorbent for all the experiment. Preparation of synthetic metal solution Stock solution of 1000mg/l was prepared using Potassium dichromate crystals. pH of the solution was adjusted using 0.5N NaOH or H2SO4. Fresh dilutions of 100mg/l were prepared from the stock solutions for each …show more content…

Different standards containing less than 100mg/L (20, 40, 60, 80, and 100) were prepared and maintained at pH less than 2. To 10ml of standard, 0.2ml of diphenylcarbazide was added as a complexing agent. The solution was incubated until red violet color was developed. The absorbance was measured spectrophotometrically at 540nm. A blank was prepared for Cr (VI) analysis. The amount of chromium present in the sample was determined from calibrated curve according to the standard method (American Public Health Association (APHA) method 3500-

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