2. FORMATION OF HYDRAZONE FROM ESTER Materials required: * The ester which was synthesized in the previous reaction. The total weight of ester obtained was 230mg. * Methanol – 20 ml * P-toluene sulfonylhydrazide (1.2 equivalent ) Procedure: * The ester was transferred in a round bottom flask and it was mixed with about 20ml of methanol and stirring was done on a magnetic stirrer till the ester dissolves in it completely. * In the above RB, a calculated amount of 1.2 equivalent amount of PTSH was added during continous stirring.
Microcalorimetry experiment was performed on Setaram−C80 heat flow calorimeter coupled to a multiport high-vacuum homemade glass manifold. A specific amount of the sample (approx. 500 mg) was taken into a sample cell, reference cell was taken as empty and together they are connected with Pyrex tee. The sample was heated from room temperature to 200°C under vacuum and kept for 2 h, then 3-4 doses of helium gas were introduced into the system to remove any excess residue such as moisture or organic impurity from the system. The system was heated for 4-5 hr under vacuum at 200°C and then cooled down to the temperature (50°C) where we want to perform the adsorption study.
The three petroleum ether extracts were combined and concentrate to its minimum volume by using a stream of nitrogen. Analysis of fatty acids methyl ester (FAMEs) was carried out by Gas Chromatography using( Hewlett Packard, Palo Alto, CA, USA) (HP 6890) and(FID) detector was used at 250 °C . The fatty acid methyl siloxane capillary column HP – 5 (30m x 0.32 mm I.D. × 0.25 μm film thickness) was used. Nitrogen was used as the carrier gas (0.8 m / min gas flow).
Given that mass was lost from the copper carbonate hydrate during heating, in this decomposition reaction, how many moles of solid product were produced? The molecular weight of the solid product is 79.545 g/mol (moles = mass / molecular weight). There were 4.183 x 10^-20 of solid product produced 4. Copper carbonate hydrate produces 1 mole of water (MW = 18.015 g/mol) was for every mole of solid product (MW = 79.545 g/mol) produced. Given that 3.327 g of product was produced during the reaction, how many grams of water were released as water vapor?
A: Yes, we need molar mass of sulfur, hydrogen, and chlorine. Then set the molar mass of copper and zinc to x and y. And use the mole ratio, which is 1:1 for two reactions to solve the unknown. 4. How many grams of zinc chloride could be formed from the reaction of 3.57g of zinc with excess HCl?
The solution was stirred at room temperature for 8h. The solvent was blown out with nitrogen. The residue was added to 1 ml of water containing 0.1% TFA and purified on RP-HPLC. Massspec of the final product clearly indicates presence of RB modified on PEI by series of peaks matching different polymer compositions (see Fig. 6).
It has a melting point of 1585K (which is the same as 1312°C 2394°F), heat of fusion of 10,05 kJ/mol, and boiling point of 3546 K( 3273 °C, 5923 °F). Gadolinium has an electron configuration of [Xe]4f 75d16s2; and vapour pressure of7.39 Pa at temperatures of 2000 K.
2.9. Estimation of Hydrogen peroxide (H2O2) 10 213 The concentration of H2O2 was determined by the method of Okuda et al (38). Fresh leaf 214 sample (0.5 g) was grounded in ice-cold 200 mM HClO4 and was then centrifuged at 215 1200 g for 10 min followed by neutralization of HClO4 of the supernatant with 4M KOH. 216 The insoluble KClO4 was eliminated by further centrifugation at 500g for 3 min. In a 217 total volume of 1.5 mL, the reaction mixture contained 1 mL of the eluate, 400 mL of 218 12.5 mM 3-(dimethylamino) benzoic acid in 0.375 M phosphate buffer (pH 6.5), 80 mL 219 of 3-methyl-2-benzothiazoline hydrazone and 20 mL of peroxidase (0.25 unit).
Two highly sensitive methods for the determination of genotoxic alkyl methane sulfonates (AMSs) and alkyl paratoluene sulfonates (APTSs) in lamivudine using hyphenated techniques have been presented by N.V.V.S.S. Raman et al. (2012). AMSs were determined by GC–MS method using GSBP-INOWAX (30 m×0.25 mm×0.25 μm) column. Temperature program was set by maintaining at 100 °C initially for 3 min, then rised to 220 °C at the rate of 15 °C/min and maintained at 220 °C for 16 min.
The XRD patterns were recorded using Rigaku Miniflex IIC Table Top XRD. The SEM images are obtained using Tescan Vega III SEM instrument. Malvern Zeta Sizer Nano series Nano-S instrument was used for Particle size analysis. 2.3 Preparation of Silver Nanoparticles 2.3.1 Preparation of silver nanoparticles by borohydride reduction method. Firstly a 100 ml solution of AgNO3 (1mM) is prepared in a beaker.
The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.
Since xylene has a high boiling point of 140 °C, the reaction proceeded speedily. Crystallization of the product took place as the mixture underwent cooling. Filtration was used to isolate the product. The tests with regards to the melting point and spectra led to the determination that 9,10-dihydroanthracene-9,10-α,β-succinic anhydride was produced. The Diels Alder reaction is a high yield reaction and can be used to form as many as 4 carbons.