The purpose of this experiment is to perform a two step reductive amination using o-vanillin with p-toluidine to synthesize an imine derivative. In this experiment, 0.386 g of o-vanillin and 0.276 g of p-toluidine were mixed into an Erlenmeyer flask. The o-vanillin turned from a green powder to orange layer as it mixed with p-toludine, which was originally a white solid. Ethanol was added as a solvent for this reaction. Sodium borohydride was added in slow portion as the reducing agent, dissolving the precipitate into a yellowish lime solution.
Abstract – Methyl trans-cinnamate is an ester that contributes to the aroma of strawberry. It can be synthesized by an acid-catalyzed Fischer esterification of a methanol and trans-cinnamic acid under reflux. The solution was extracted to obtain the organic product, and evaporated residual solvent The yield was 68%, but there is some conflicting data regarding the purity. The melting point, IR, GC-MS indicate a highly pure desired product whereas 1H NMR shows there are unreacted reagents still present.
In order to achieve separation within the compound trimyristin from ground nutmeg seeds, employing a process known as extraction, a process of separating a single component to isolate and purify chemical compounds from a mixture. There are two types of extractions, solid-liquid, and liquid-liquid. The separation in this experiment was conducted through not only filtration to separate and purify the triglyceride trimyristin from the nutmeg, simple distillation to remove the solvent from the product, but also the suction filtration to isolate the crystals. By using the solvent, dichloromethane the extraction of trimyristin isolated the crude oils from nutmeg and then acetone was used to help in dissolving the crystallized product. Unfortunately, my material did not come out correctly, it turned out to be a very waxy solid that was not able to be recrystallized, no matter how much acetone I used to change the texture, it still remained the same.
Results and Discussion 3.1 Characterization of Baclofen 3.1.1 Baclofen melting point: The measured melting point of Baclofen was found to be 207°C.This result is the same as reported in references, which indicates the purity of the drug powder used in the study (27). 3.1.2 Baclofen λ max: Scanning of Baclofen solution (100μg/ml) in phosphate citrate buffer (pH 7.4) by UV spectrophotometer at 200-400 nm gave the spectrum Shown in figure () .The maximum absorbance (λ max) found to be 220nm, which is similar to standard references (36, 37).
Determination of the molar mass of magnesium Aim To determine the molar mass of magnesium with the ideal gas equations and Dalton’s law about partial pressures. Materials and chemicals gas measuring tube 50.0cm3 graduated cylinder 1000.0 cm graduated cylinder 10.0cm3 cork with a hole copper wire thermometer barometer magnesium ribbon HCL 35% (concentrated) Background theory Dalton’s Law of Partial states that the total pressure of a gas is equal to the sum of pressure of each individual gas at a specific temperature. The combination of all the partial pressures of the gases that make up the atmosphere gives the total pressure of the atmosphere. The average pressure of the atmosphere is 101 kilopascal(KPa).
The materials and methods used in this experiment are as written from the Laboratory Supplement of David L. Shultz. To properly prepare for the experiment, the materials needed were one stoppered shell vial or small Erlenmeyer flask for flies, seventy flesh flies (Sarcophaga bullata, ice-cold to anesthetize them), one wire test-tube rack (small mesh), a few paper towels to cut the flies on, one razor blade, one enamel pan, one thermometer, one grease pencil, one piece of plastic wrap, one large plastic beaker filled with crushed ice, seven large glass test tubes to hold reagents, homogenate, and centrifuged fractions, seven glass reaction tubes (small size, about three inches tall), and seven plastic pipettes (one milliliter and controls). When all
Abstract — This experiment was conducted to familiarize the students with the procedures regarding distillation—to be more precise, the separation of ethanol from an alcoholic beverage—using a distillation set-up consisting of boiling chips, a Bunsen burner, a condenser, a thermometer and several other materials. In the end, it was discovered that one may actually separate a homogeneous mixture, given that the components of said mixture differ in volatility and that they utilize a complete distillation set-up and follow laboratory safety rules and regulations. Keywords — Matter, homogeneous and hetereogeneous mixtures, distillation, volatility, boiling point I. INTRODUCTION There are typically two categories of matter, these are pure substances
The basic principle for the synthesis of kestoses involves the action of invertase on sucrose. Invertase hydrolyses sucrose, producing glucose and fructose. Invertase also plays a very important role in kestose synthesis: It transfers the fructosyl residue (resulting from hydrolysis of sucrose) onto the fructose residue of sucrose (1- and 6- kestose) or the glucose residue of sucrose (neo-kestose). In this way, the respective kestoses (1, 6 and neo) are created. The method used for the synthesis of the kestoses was adapted from Gross (1962).
Aim The aim of the experiments to be carried out is to determine the kinetic parameters, Km and Vmax, of Alkaline Phosphatase. Theory, Principles and Application of Principles Enzymes are a huge varying group of proteins which are needed to carry out essential metabolic functions in cells.