Preparation of ketoconazole loaded Proliposomes Ketoconazole (KTZ) loaded proliposomal gel formulations were formulated by method reported by Perret et al. 1991 with slight modification. Constant amount of drug was added to varying ratios of phophatidylcholine and cholesterol which constitute lipid component of 1mmol quantity. This lipid mixture was prepared in clean and dry, wide mouthed glass vials to which 400µL of absolute alcohol was added and after confirming the formation of homogenous dispersion, the glass vials were heated thermostatically on a water bath at 60-70oC with intermittent shaking. Add 160µL of double distilled water maintained at the same temperature to the transparent solutions formed, these upon cooling change to yellow translucent liquid/gel or white creamy proliposomal gel.
Carbon dioxide and water in the solution were also clear. Once the solution was completely titrated, Mn7+ ions remained unreduced and changed the color of the solution to pink. The KMnO4 was added to each solution until the oxalate solution reached the end point and changed to an extremely light pink color. The change in volume in the burette of the potassium permanganate recorded in all three trials was used to calculate the moles of oxalate in the 0.100-gram compound, giving the percent composition of the compound. The three trials reacted 27.95 mL, 26.61 mL, and 25.74 mL of potassium permanganate to determine 55.7%, 53.0%, and 51.3% respectively of oxalate in the compound with a 53.3% average.
Procedure A. Preparation of NaOH solution The molarity of a solution is the ratio of the number of solutes dissolved in a liter of solution. To figure out the needed mass (in grams) of NaOH pellets to be dissolved in a 0.25 L of water, remember that a mole is equivalent to the quotient of mass over the molar mass of the substance. This was used to rearrange the base formula and to derive the mathematical equation of mass in terms of molarity. mass (g) = (Molarity)(Volume)(Molar mass) The pellets were dissolved thoroughly then was used in filling up the 100 mL volumetric flask.
Results and Discussion 3.1 Characterization of Baclofen 3.1.1 Baclofen melting point: The measured melting point of Baclofen was found to be 207°C.This result is the same as reported in references, which indicates the purity of the drug powder used in the study (27). 3.1.2 Baclofen λ max: Scanning of Baclofen solution (100μg/ml) in phosphate citrate buffer (pH 7.4) by UV spectrophotometer at 200-400 nm gave the spectrum Shown in figure () .The maximum absorbance (λ max) found to be 220nm, which is similar to standard references (36, 37). As shows in figure (3-1). Figure (3-1): UV scan of Baclofen at pH 7.4 shows the λ max at 220 nm 3.1.3 Baclofen calibration curve: Figure (3-2) shows the calibration curve of Baclofen in phosphate
This was approached by finding the molar mass of sodium thiosulfate pentahydrate and then using that value to convert the grams of the sodium thiosulfate used for the initial creation of the weigh bure into moles. The molar mass of sodium thiosulfate pentahydrate was found by adding the atomic masses of each element found in the compound, as shown in Example 1. The molar mass was then used to convert the grams used into moles by using dimensional analysis. Example 1: Na2S2O35H2O (22.990Nag x 2)+(32.066Sg x 2)+(15.999Og x 3)+(1.008Hg x 10)+(15.999Og x 5)= 248.18 g/mol Example 2: 0.21gx1 mol248.18 g= 8.46 x 10-4 mol The last step in completing the Preparation Table was to calculate the concentration of the standard thiosulfate solution by dividing the moles of sodium thiosulfate pentahydrate by the mass of the solution, in grams(Example 3). Example 3: 8.46 x 10-4 moles of Na2S2O35H2O/ 9.70 grams of solution = 8.72 x 10-5
In addition, phenolphthalein was added as an indicator. The aliquots were titrated against sodium hydroxide (NaOH) solution until end point was reached, after which volume of NaOH consumed was recorded. The value of the rate constant, k, obtained was 0.0002 s-1. The experiment was then repeated with 40/60 V/V isopropanol/water mixture and a larger value of k = 0.0007 s-1 was obtained. We concluded that the rate of hydrolysis of (CH3)3CCl is directly proportional to water content in the solvent mixture.
Absorption was read by spectrophotometer at wave length (510CHOL) and (505TG). 6. After this we applied the following equation Result = ×CON. standard Extraction of tissue The extract one gram of fat tissue using n hexane, resolve to dissolve tissue homogenizer homogeneity by adding 1 ml of the same solution to become a 3: 1 and continued homogeneity of the sample to become a solution homogeneity. The solution homogeneity expelled, by centrifugation for 10 min.
0.5 mL of AuNPs solution was added to the above mixture. The UV-Vis spectra were recorded with a time interval of 1 min in a scanning range of 200-600nm at ambient temperature (25±20C). Antimicrobial activity The agar disc diffusion method was employed for the determination of antimicrobial activity of the papaya leaf extract stabilized gold nanoparticles. The 0.1 ml of 108cfu/ml of different pathogenic bacteria suspension was spread on different plates nourished with LB media. Filter paper
5-aminotetrazole monohydrate: In a 250 ml round-bottom flask equipped with a condenser for refluxing (90 °C) and a magnetic stirring bar, 5.00 g (5.95 mmol) dicyandiamide (three times crystallized), 7.47 g (11.9 mmol) sodium azide and 11.00 g (17.8 mmol) boric acid and 100 ml of water is added and allowed to reflux for 24 hours, after the completion of the reaction, until the solution pH to about 2 to 3 as hydrochloric acid 37% is added (about 12 ml) Then the reaction mixture was cooled in a refrigerator for 18 hours and the white crystals formed. The mixture was filtered and washed three times with 10 ml of water and and dried in 60 °C for 5 hours and finally 45.8 g of product by it will be obtained. 5-Aminotetrazol monohydrate: Yield:,