Harvest And Fix Spheroids Research Paper

We then added 10cm3 ethanoic anhydride to the salicylic acid and swirled the contents, this mixes together the two chemicals. We then added 5 drops of concentrated sulphuric acid to the flask and thoroughly swirled the mixture, this creates the solution that makes the aspirin. We then warmed the flask for 20 minutes in a 400cm3 beaker of hot water which was approximately 60°C, we made sure the flask did not go above 65°C because this could have caused the contents to evaporate. Part 2: Using a 25cm3 measuring cylinder we measured out 15cm3 of ethanol into a boiling tube and then prepared a beaker half filled with hot water at approx. 75°C, we got this temperature by filling the beaker with cold water and slowly adding boiling water from a kettle until we reached the right temperature.

Experimental Methods: 1. SYNTHESIS OF 4-BENZOYL BUTYRIC ACID METHYL ESTER Materials required * 5 oxopentanoic acid : 2 gm (Aldrich) * Methanol : 50 ml * Acetic Acid (Rankem) Procedure: * 2 grams of 5 oxopentanoic acid was weighed and placed in a round bottom flask and then to it 50 ml of methanol was added. It was placed on a hot plate and the temperature was increased to 50 degrees under ambient air conditions.

This study was conducted with a partner, since some parts of the experiment were able to be done simultaneously. One partner prepared a TLC developing jar by pouring a small layer of 4:1:1 propanol/acetic acid/water into a developing jar. A solvent wick was made by wetting a piece of filter with the solvent, and it was placed in the jar. A silica coated TLC plate was obtained, and a spotting line was carefully drawn approximately 1.5 cm from the bottom of the plate using a pencil. Extra care was taken to not touch the plate with bare skin.

Ignite with a meker burner for about 1 hour. Complete the Ignition by keeping in a muffle furnace at 500 °C to 570 °C until grey ash results. Cool and filter through whatman filter paper No. 42 or its equivalent. Wash the residue with hot water until the washings are free from chlorides as tested with silver nitrate solution and return the filter paper and residue to the dish. Keep it in an electric air oven maintained at 135 ± 2 °C for about 3 hrs.

5-aminotetrazole monohydrate: In a 250 ml round-bottom flask equipped with a condenser for refluxing (90 °C) and a magnetic stirring bar, 5.00 g (5.95 mmol) dicyandiamide (three times crystallized), 7.47 g (11.9 mmol) sodium azide and 11.00 g (17.8 mmol) boric acid and 100 ml of water is added and allowed to reflux for 24 hours, after the completion of the reaction, until the solution pH to about 2 to 3 as hydrochloric acid 37% is added (about 12 ml) Then the reaction mixture was cooled in a refrigerator for 18 hours and the white crystals formed. The mixture was filtered and washed three times with 10 ml of water and and dried in 60 °C for 5 hours and finally 45.8 g of product by it will be obtained. 5-Aminotetrazol monohydrate:

Task 1 M1 Describe the scientific principles behind each of the three procedure above. Vacuum filtration is a procedure when a sold needs separating from a solvent to react the mixture. Then the mixture of a solid is measured through the filtration paper in a Buhner funnel. The liquid is drained through the funnel into the flask.

The mixture was transferred to a separatory funnel, separated into an organic layer and water layer, and then drained. The water layer was washed twice with 10 mL of hexane. The organic layer was dried

For TLC profiling, 4 TLC plates were prepared for the testing of each solvent. As shown in Figure 1, the green food dye was placed at the bottom center, specifically 0.5 cm away from the bottom of the plate, with the use of a capillary tube. Each one of the silica plates were then vertically placed in a small beaker with its inside surrounded by a filter paper saturated with the solvent to be tested and a small amount of the same solvent at the bottom. The TLC plate was then taken out when the rising solvent was about to reach the top of plate. The ammonia: 1-butanol solvent was tested 7 times due to some personal

Shake funnel and rinse off water layer ( This contains the sulfuric acid and majority of methanol). Again wash the ether with 25ml of water and then wash the organic layer with 25ml of 10% sodium bicarbonate to extract unreacted benzoic acid. Again shake separatory funnel with frequent venting of pressure and opening the stopcock. Allow the separation of layers and drain off bicarbonate layer into a beaker. Wash ether layer with saturated sodium chloride solution and retain ether layer.

When the gel was polymerized, isopropanol was removed on the top of the running gel with water and thus, water was also removed as well. Then, stacking gel was prepared and its polymerization reaction was started with TEMED, and its solution was quickly poured on the top of the running gel. Therefore, a comb was inserted into the stacking gel before its polymerization, to form the loading wells. When the gel was polymerized, the inner chamber was filled with electrophoresis running buffer and the outer chamber was filled half. Chemical modification of ERAB (Wild Type and Mutant) Protein with Fluorescein Maleimide Wild type and mutant ERAB

The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.

After that, put an aluminum wire into the beaker, and after a certain period of time the solution gains color. To finish the reaction, 5 drops of 6M of Hydrochloric acid is added into the beaker to clean the solution, which means that acid dissolves all salts of aluminum that is on the solution. After finishing the chemical process, collect and use the Butcher funnel to wash the cooper because it is going to be used to a vacuum filtration. After finishing the filtration, measure the weight of the sample and dry it. Finally, Do again these two steps until notice that the subtractions of these masses are about 0.005 g, and then arrange all the chemical

The chloroform and caffeine mixture was collected and into a conical flask labeled A. The remainder of the solution was discarded. This was repeated for beakers B and C. 9. Sodium sulphate was then added to each beaker to dry the liquid by getting rid of any remaining water from the solution. The sodium sulphate was then filtered and discarded.

Two chemical reactions are carried by adding sodium hydroxide to the acidic solution from Part I. During the first reaction is the neutralization of the excess of nitric acid in the mixture by sodium hydroxide. The second reaction takes the place after naturalization is a complete and NaOH is in excess. While the liquid inside the beaker is being stirred, with the stirring rod, 10 ml of 6 M NaOH is poured into the solution from Part I at 1 mL at a time. After each 1 mL the solution is tested for acidity with red litmus paper.

Category: Chemistry Experimental Question: How does the temperature of the environment affect the growth of crystals in fudge? Hypothesis: If crystallized fudge is cooled under two different temperatures, then the lower temperature will grow bigger crystals than the high temperature, because solubility in cold temperatures is lower. Why fudge was chosen: Crystals form in igneous rocks, and this experiment was chosen to explore the sizes of crystals formed under different conditions. The topic was decided upon because crystals are formed under extreme conditions, and it would be helpful to know under which conditions do they grow the largest. The information would be helpful to know because if anyone decided to grow crystals on their own, they