Hb1c Lab Report

able kits ((On Siemens Dimension® clinical chemistry analyzer -RXL-and X pand, Germany). Flex® reagent cartridge: HB1C

SCOPE:

HbA1c reflects the average blood glucose level during the preceding 2 to 3 months. HbA1c is thus suitable to monitor long-term blood glucose control in individuals with diabetes mellitus.

SAMPLE REQUIREMENT: Type of specimen: K3EDTA Anticoagulated whole blood. The samples must be free of clots. They have to be mixed thoroughly and analyzed subsequently as “STAT”.

PRINCIPLE OF TEST: The Dimension®(RXL-and X pand) HB1C assay measures both HbA1c and total hemoglobin.

The HbA1c measurement is based on a turbidimetric inhibition immunoassay (TINIA) principle, and the measurement of total hemoglobin

This reagent lyses the red blood cells and simultaneously converts the released hemoglobin to a derivative that has a characteristic absorbance spectrum. An aliquot of the lysed whole blood is transferred from the first cuvette to a second cuvette where total hemoglobin concentration is measured at 405 nm and 700 nm.

Whole blood + lysing reagent Released Hemoglobin Haemoglobin derivative (Measured at 405 nm)

Haemoglobin A1c Measurement: The same aliquot of the lysed whole blood that is transferred from the first cuvette to the second cuvette for the Hb measurement is also used for the measurement of HbA1c.

The second cuvette contains an anti-HbA1c antibody in a buffered reagent. Hemoglobin A1C in the sample reacts with anti-HbA1c antibody to form a soluble antigen-antibody complex. A polyhapten reagent containing multiple HbA1c epitopes is then added to this cuvette.

The polyhapten reacts with excess (free) anti-HbA1c antibodies to form an insoluble antibody-polyhapten complex. The rate of this reaction is measured turbidimetrically at 340 nm and blanked at 700 nm and is inversely proportional to the concentration of HbA1c in the

SAMPLE REQUIREMENT: Type of specimen: Serum (Blood collected in Plain tube and centrifuged to separate serum).

PRINCIPLE OF TEST:

In the presence of a strong base such as NaOH, picrate reacts with creatinine to form a red chromophore. The rate of increasing absorbance at 510nm due to the formation of this chromophore is directly proportional to the creatinine concentration in the sample and is measured using a Bichromatic (510,600nm) rate technique. To correct for non-specific reaction caused by serum/plasma pseudo-creatinine chromogens, including proteins and ketones, the results for serum or plasma are corrected by -18 μmol/L (-0.2 mg/dL).(2, 4, 14-18) Alkaline pH (NaOH)

Creatinine + picrate red chromophore (absorbs at 510nm)

EQUIPMENTS & APPARATUS:

Siemens Dimension® clinical chemistry analyzer (X pand and RxL Max)

Flex reagent cartridge, Assay Cups, tips, pipettes.

Flex – CREA

REAGENT REQUIRMENTS:

Reagent