Record results to determine if NaHCO3 increases or decreases the pH of the water. 10. If NaHCO3 increases the pH of the water, it shows that it will increase the pH of the blood and if NaHCO3 decreases the pH of the water, it shows that it will decrease the pH of the blood. 11. Add 30ml of 0.1M HCl into a beaker containing distilled water.
The line of best fit gives the respiration rate of day-old seedlings as the concentration of NaCl they are exposed to increases. As NaCl Concentration increases the rate of cellular respiration decreases by .108 ppm CO2/g per second. This overall decrease throughout the data further supports our hypothesis. Discusion: The data collected in the experiment does support our hypothesis. By examining the data as a whole a trend of decreased cellular respiration in seedlings soaked in solutions with increased NaCl concentrations.
Moreover, it can be seen that the cooling capacity could gain 685.4 W when Tg = 65 °C and 482.8 W when Tg = 55 °C. With the increase in generating temperature, the cooling capacity decreased at first and then increased when the generating temperatures ranged from 40 °C to 70 °C. The experiment was repeated under the same conditions. Figure 4 illustrates the effect of the generating temperature on the system COP, cooling capacity and critical condensing temperature of the steam ejector when the generating temperatures ranged from 60 °C to 70 °C. As shown in Fig.
8. The results in the figure reveals that the reduction efficiency decreases with increasing in the flow rate of the dye solution. Almost 13% of MB was removed at flow rate 15 mL/min, then it is increased to 75% for flow rate 25 mL/min, where as 33% and 20% of MB was removed at flow rate 40 and 70 mL/min, respectively. The role of increasing the degradation rate of pollutant with increasing the flow rate is valid up to a certain limit then the reaction becomes slower. These phenomena could be illustrated by the fact that once the flow rate was slow MB in the sample solution got more contact time with UV radiation induced.
Clean up and return the materials. Paragraph 3 In my bar graph I presented the relationship between the temperature of water and an Alka-Seltzer’s rate of reaction time when dropped into the water. The data proves that if the temperature of water increases, then the reaction rate of an Alka-Seltzer tablet will increase as well. I presented the data of the temperatures; 21°C, 41°C, and 28°C for each four trials and their averages. The graph presents the trend of the rate of reaction decreasing when the temperature is lower.
The aim of the investigation was to determine the effect of pH on the rate at which catalase decomposes hydrogen peroxide and consequently answer the researchable question “How does pH influence cells and consequently an organism”. The hypothesis, “As the pH deviates from 7 the initial rate of oxygen production will decrease” is supported by the results. The trend displayed in Figure 3 is, as the pH deviates from 7 the initial rate of reaction decreases. Figure 3 shows that the rate of reaction (%O2/s) decreases substantially as the pH increases and decreases to 8 and 6 respectively from a pH of 7. This is indicated as the graph shows that the initial reaction rate for pH 7 was 0.143 %O2/s compared to 0.047 and 0.053 for pH 6 and 8 respectively.
That means that there was a small lag phase. As the initial crude oil concentration increased to 20 and 40 ml/L the lag phase raised. In fact, it was found that the lag phase was increased when the initial crude oil concentration was higher due to the slower cell adaptation. This shows that the toxicity of crude oil inhibited MS1 strain at high concentrations (19, 33). Varius types of kinetic substrate consumption and inhibition models have been used to explain the dynamics of microbial growth on different compounds for example Phenol, Toluene, Benzene and p-cresol (16, 32, 34).
If the temperature, pH and enzyme concentration is kept constant then the rate of reaction will start to decrease as well as the hydrogen peroxide concentration. Aim: To investigate the effects of changing the concentration of the enzyme catalase that it has on the rate of breaking down the Hydrogen Peroxide solution. Dependant and Independent Variables: The Dependent Variables: Amount of time it takes when the bubbles start to rise till when they stop. The Independent Variable: Amount of Hydrogen Peroxide solution. The Controlled/ Fixed Variables are: • The amount of hydrogen peroxide inserted in each test tube.
Separation of Liquids by Fractional Distillation and Analysis by Gas Chromatography Methods and Background This lab was exceptionally knowledgeable and important in order to understand how certain compounds can be separated based on their boiling points (Landrie, 43). This experiment in particular focuses on understanding the separation of 1:1 mixture of acetone and 1 propanol using the method of fractional distillation (Landrie, 43). In the previous experiment, we focused on understanding the same separation but in terms of simple distillation. If compared with the data shown below with the data in the previous experiment, overall, the fractional distillation showed better and more effective results (Landrie, 43). This experiment also provided better
The enzyme would be inactivated. 2. The catalase which is the enzyme breaks down the substrate hydrogen peroxide forming the product water and oxygen. There is less hydrogen peroxide concentration as time is increased due to break down of the substrate. As time is increased more oxygen is given off, more water is formed resulting in a decrease in color intensity.
As seen in the trend of both buffer, once the pH is lower than 3, the slope of dv/dpH increase drastically, showing the decreasing effects of the buffer. On the other hand, in the trend of both buffer on the right side of graph shows when NaOH is added, the change in pH is more drastic once past about pH 5. Although buffer 1 and buffer 2 shows a similar trend, the plot of buffer 1 is above the plot of buffer 2. The reason for this is that buffer 1 is made by an acid and base with an almost equal concentration. This makes buffer 1 a greater buffer compared to buffer 2.
The titrations were all a bit different and that was because they all contained different liquids. The first titration, acid into water, showed a noticeable drop in ph after approximately 5.5 mL of acid was added and was consisted in showing low numbers. This determined that the first hypothesis, stating that the pH would go up when acid was added into water, was false. Dissimilarly to the second titration, base into water, the pH showed a noticeable increase after around 10 mL of base was added and once again, remained consistently high in pH. The third titration, acid into liver homogenate, the pH decreased a bit at 8 mL of acid, but remained fairly consistent at 12 mL.
As a result, the C value dropped from 1.2 to 0.3 using carbon working electrode plate. Since, the absorbance and the c value had been raised, the sensitivity of the assay compared to ELISA was expected to decrease. However, by measuring the precision profile of 0.0125 µg/ mL Antibody concentration and the conjugate of dilution (1:15,000), the LOD of 14 ng/L using electrochemical detection was obtained compared to 10 ng/L using
Because this pH was too acidic in comparison to its optimal range, the enzyme began to denture and was rendered inactive. As the pH grew closer to the optimal pH range, the reaction rates began to drastically increase, which is shown through the results provided from pH 3, 6, 7, and 8. However, pH 6, 7, and 8 were the most consecutively similar, and would therefore mean that the catalase optimum ranges from these three. From these levels, pH 8 provided the highest reaction rate of 59 mL/minute. From this data, it can be decided that this is the optimal level for the catalase.
Nitrate reduction in the gram negative bacteria was conducted and when bacterium produce nitrate reductase when grown in a medium containing nitrate, the enzymes will convert the nitrate to nitrite. If nitrite is present the medium will turn red. This indicates a positive test (Goldman). If the bacterial is one of the few specials that can produce the enzyme Urase, this will be the key test that will separate this gram negative from all of the other possible candidates. Narrowing down the unknown microorganism to gram negative, this approach was helpful to take the next step, in some bacteria the cell wall is surrounded by cell enveloped called capsule, also some bacteria make capsule when faced in a harsh environment to protect them.