Standardized Extraction Methods

Traditional herbal medicine and their preparations have been widely used for the thousands of years in developing and developed countries owing to its natural origin and lesser side effects or dissatisfaction with the results of synthetic drugs. Extraction, as the term is used pharmaceutically, includes the partition of restoratively dynamic bits of plant or creature tissues from the latent or inactive segments by utilizing specific solvents as a part of standard extraction methodology. The items so acquired from plants are generally sullied fluids, semisolids or powders planned just for oral or outside use. The reasons of standardized extraction methodology for unrefined medications are to accomplish the remedially craved part and to wipe

The yield of chemical extraction depends on the type of solvents with varying polarities, extraction time and temperature, sample-to-solvent ratio as well as on the chemical composition and physical characteristics of the samples [4]. In general acetone, water and methanol were commonly used as extraction mediums [5], In particular, methanol has been generally found to be more efficient in extraction of several compounds [6-9]. This might be due to the high polarity index of (5.1) Methanol. The bioactive compounds from plants belong to various chemical groups such as tannins, alkaloids, glycosides, lignins, terpinoids, etc., with various polarities and also with certain groups of non polar compounds. The non polar compounds also have the capacity to fairly soluble in methanol. The previous studies reported that methanol is a better solvent for more consistent extraction of antimicrobial substances from medical plants compared to other solvents, such as water, ethanol, and hexane [10, 11]. Thus this research aims to develop a standard extraction procedure using methanol as an extraction medium, with respect to the selected

However, to the best of our knowledge, optimization of extraction of active components from fruits of Terminalia Chebula using RSM has not been reported yet. Therefore, the objective of this study was to determine the best extraction conditions for Terminalia chebula fruits, in order to maximize the yield of active compounds by using response surface methodology. In this work, the various levels of immersion time of Terminalia chebula in methanol, with different sonication time and levels of temperature were selected based on the previous experience in the extraction [18]. The response surface methodology, Box and behnken designs were adopted with three different levels and variables to analyse the optimum condition for the better and improved odour reduction in terms of zone of inhibition. Unlike the conventional empirical method, RSM can take into account the possible interrelationship among the test variables while minimizing the number of experiments [19]. Thus, it is a powerful tool that can provide a complete optimal condition to improve a process. With this kind of approach, it is possible to create response surfaces that allow the ranking of each variable according to its significance on the studied