Herbal Extraction Analysis

The antibacterial effect of plant extract majorly depends on the process parameters of herbal extraction. In this aspect, this study focused to optimize the extraction conditions for better performance after application. The effect of variable like extraction timing, sonication time and the extraction temperature were taken as key parameters. The Box and Benkhen design was adopted to study the effects of this parameter on the effectiveness of extraction in terms of in-vitro antibacterial activity of treated textile (in Zone of inhibition) against odour causing bacterial strains. The response surface methodology results showed that the increment in the extraction time increases the antibacterial activity from 12 hr to 36 hr. The extraction temperature

The bacterial strains like Corynebacterium Sp (MTCC 8730), B. licheniformis (MTCC 429), M. luteus (ATCC 49732), Corynebecteriurm acnes (MTCC1951), were used based on their importance in odour formation characteristics on human axilla.
METHODS
Preparation of Herbal Extracts and application
The methanol extract of the powder was obtained. 10000 mg of powder was soaked in 100 mL of methanol separately for 24 h to obtain 10% concentrated solution; the active substances were dissolved in methanol. The extract was filtered and used for the further processes. The methanol extracts of Terminalia chebula were applied to the cotton fabric by dipping in the bath with material-to-liquor ratio of 1:10 and then pad-dried at room temperature [20].
In-vitro Antibacterial studies
Agar Diffusion Method (SN 195920: 1992)
The treated and untreated fabric samples were placed in the AATCC bacteriostasis agar (AATCC, 2005), which was previously inoculated (mat culture) with a test organisms. After incubation, a clear area of uninterrupted growth underneath and along side of the test material indicates the antibacterial effectiveness of the fabric. The area of the inhibition is a qualitative measure of antibacterial activity