Liquid Chromatography Lab Report
Name :SiphesihleGamede
Student number : 213571124
Experiment : #10
Date : 4 March 2015
Experiment Name : High Performance Liquid Chromatography (HPLC), Quantification of caffeine in various drinks
Aim
The aim of this experiment was to ascertain the concentration levels of caffeine in cola soft drink samples such as coke, monster and red bull by utilising the internal standard method using HPLC. The experiment was also aimed at investigating the use of solid phase extraction for sample clean-up.
Figure 1.1 shows a skematic diagram of an HPLC system. Figure 1.2 shows a skematic diagram of a solid phase extraction system.
Introductory Questions
Define SPE and explain the role of each of the steps used to prepare the SPE cartridge for the isolation of the analyte.
Solid phase extraction (SPE) is an extraction method that uses a liquid and solid phase to isolate a single analyte or a specific type of analyte from a solution. It is usually used to clean up a sample before using a chromatographic or other analytical method to quantify the amount of analyte(s) in the sample.
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One of the reasons why SPE is a suitable technique for this analysis is the fact that there should be higher recoveries (% recoveries)since both caffeine and theophylline are polar compounds, with theophylline being more polar than caffeine which tells us that caffeine will have the longer retention time than theophylline. Other reasons include advantages such as SPE provides cleaner extracts and is easier to automate compared to other extraction …show more content…
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