Holarrhena Antidysenterica Research Paper

Key words: Holarrhena antidysenterica, methanolic extract, analgesia, tail flick test, hot plate test
Abstract
The seeds and bark of Holarrhena antidysenterica are reported to have antidysenteric, antibacterial, astringent, anthelmintic, stomachic, febrifugal properties. In the present study, the analgesic activitiy of methanolic extract of the leaves of Holarrhena antidysenterica was investigated. Analgesic activity of methanolic extract of leaves of Holarrhena antidysenterica was performed in mice at three doses 100, 200 & 300 mg/kg bw using tail flick and hot plateassay methods. In Hot plate test, the extract at a dose of 100 mg/kg bw showed significant analgesic effect at 30 and 60 minutes exposure. The response times were higher than

The experimental plant is available in wasteland and road side. The plants were identified at the Bangladesh National Herbarium institute, Mirpur, Dhaka, Bangladesh. The collected leaves were washed with tap water and dried in shade for about ten to fourteen days. Then air-dried leaves were grounded and preserved (at 20°C- 25°C) in closed container.
Preparation of Plant Extracts
About twenty grams of the fine powder of plant leaves were weighed. Then the powder was soaked by using 250 mL of methanol into an Erlenmeyer flask. The solution was covered and occasionally shaken and allowed to stand for three days in room temperature. After shaking and filtration through Whatman filter paper (No.1), the solvent was dried by using a rotary evaporator under reduced pressure at temperature below

Here, the analgesic activity of the methanolic extract of leaves was evaluated by using Eddy’s hot plate method (Eddy and Leimbach, 1953; Ghosh, 1984; Kulkarni, 1999). Five groups (6 animals in each group) were treated orally as follows: saline 0.1 mL/10g bw for the control group, diclofenac sodium 50 mg/kg bw for the reference group and methanolic extract of leaves at dose 100mg/kg, 200mg/kg and 300mg/kg respectively (3 test groups). In this experiment, the hot plate was maintained at 50±0.5°C. Animals were placed into the hot plate chamber. The time of latency period between placing the animals on the hot plate surface, and licking back paw or jump off was recorded. The latency of response time was taken as the index of antinociception and was determined at 0, 30, 60, 90 minuntes of the administration of test drugs and standard. In order to minimize the damage on the animal paws, the cut-off time was taken as 20