I) Research Question: To what extent does light effect the rate of photosynthesis in green spinach leaves? II) Hypothesis: If the leaf is exposed to light in the presence of a Carbon source, then the leaf disks float to the surface of the solution due to an increased rate of Oxygen (O2) production through photosynthesis. III) Hypothesis Explanation: when light is absorbed by leaf pigments such as chlorophyll A or B, electrons within each photosystem are boosted to a higher energy level. Leafs then produce Adenosine Triphosphate (ATP) to reduce NADPH (ferredoxin), and incorporate Carbon dioxide (CO2) into organic molecules in a process called carbon fixation. When leaves are put in the water and undergo the process of the light-dependent reaction,
From the Unknown tube professor Cooper gave me, I scratched a little on the slant surface with the sterilized inoculating loop. Then I place it on a clean prepared slide which already had a slight drop of water. The two substances are mixed together in the middle of the slide and let dry completely. One extra step of “heat fix” is necessary to adhere everything to the surface of the slide. To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water.
Step 5: Take 10 leaves of the non-shaded plant and use the scissors and the pestle and mortar to crush the leaves as much as possible. It is essential to use the leaves when they are as fresh as possible, do not leave them unattended after the measurements are taken. Step 6: Place the crushed leaves into the bottom of the glass jar of isopropyl alcohol so that they cover the bottom. Step 7: Place the lid on the glass jar tightly. Step 8: Pour hot water into a large bowl and place the glass jar in it.
Infiltrate the leaf disks and solution into each syringes. a. Remove the piston or plunger and place the same amount of leaf disks into both syringe barrel. Replace the plunger being careful not to crush the leaf disks. Push on the plunger until only a small volume of air and leaf disk remain in the barrel.
5. Join the side arm flask to source of vacuum. While dealing with this experiment you should always use thick walled tubing, the pressure will collapse with the Tygon tubing. 6. Wet the filter paper with a tiny amount of solvent to be used in the filtration.
Title: How Different Colored Lights Affect the Growth of Plants Introduction: This experiment had multiple objectives. The first objective was to establish what colors of the light spectrum photosynthesis uses. The second objective was to determine which colors in the light spectrum help a plant to grow faster, and which slow the growth of a plant down. In photosynthesis, light energy from the sun is absorbed and reflected. When a plant has green leaves, the leaves appear green because the pigments inside the plant reflect the green light into our eyes and absorb the other colors in the light spectrum.
Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture. The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used.
This was repeated until no more gas was released. Next, the funnel was suspended through a ring, and 10 ml of 5% sodium hydroxide was added. When the two layers were separated in the separatory funnel, the aqueous layer was identified. The two layers were then separated into two different beakers. The water layer was acidified by adding concentrated hydrochloric
With the use of the same flat brush, apply Untamed towards the center of the lid, although allowing room to place Fortune Teller directly in the center. Blend out any harsh lines with the use of a clean soft dome brush. 5. Line the lower lash line with Showtime with the use of a pencil brush. Clean off the brush, and then use it to add a pop of Magic Act to the inner curve to show
Wash ether layer with saturated sodium chloride solution and retain ether layer. In a small 125ml Erlenmeyer flask, dry the ether solution over anhydrous calcium chloride. Add sufficient calcium chloride so that it no longer clumps to pellets added earlier on the bottom of the flask. Remove the solvent using a rotary evaporator and weigh product. Results 1 mole of benzoic acid (C6H5COOH = 122.12grams) reacts with 1 mole of methanol (CH3OH = 32grams/mole) to produce 1 mole of methyl benzoate (C6H5COOCH3 = 136.15grams) and 1 mole of water.