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Hydrogen Peroxide Experiment

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The aim of the experiment is to investigate the catalytic effect at different conditions on the hydrogen peroxide decomposition. Hydrogen peroxide which have a chemical formula of (H2O2) is the product of metabolism that must be decomposed. Varies of enzyme catalyst (catalase) and inorganic catalyst (manganese dioxide) were used to study the effects. The effervescence occurs showed that the presence of oxygen gas. The oxygen gas collected was tested by using glowing wooden splinter test. In this experiment, the constant variable is the volume of hydrogen peroxide. 10 % of 5cm3 hydrogen peroxide is used in all boiling tubes in this whole entire experiment. The independent variable of the experiment is the use of different catalytic conditions. …show more content…

When hydrogen peroxide is poured into the test tubes, it is observed that effervescence occurs in both test tubes and bubbles are produced. This means that decomposition of hydrogen peroxide happened. However, the effervescence and bubbles produced in test tube containing fresh liver is more as compared to test tube containing fresh potato. This indicates that more cellular respiration take place in fresh liver compared to fresh potato cubes. When tested with glowing wooden splinter, the flame of glowing wooden splinter rekindled and burns brightly in test tube containing fresh liver while the glowing wooden splinter glows brightly and white fumed is formed in test tube containing fresh potato cubes. Liver is an active tissue which carry out detoxification contains more enzyme catalase. Metabolic rate of animal is higher than metabolic rate of plants. Therefore, more hydrogen peroxide are produced as by-product in animal cell as compared to plant cell. More hydrogen peroxide could bind into the active site of enzyme catalase, thus large amount of products formed. Large amount of enzyme catalase lowers the activation energy, thus speeding up the rate of conversion of hydrogen peroxide to oxygen and water. Whilst, the fresh potato cube is storage tissue which have a lower respiration rates and there is no need for a high catalase activity. Thus, less enzyme catalase in the potato cube. The hydrogen peroxide bind to the active site of enzyme in potato cube is lesser than in fresh liver. More activation energy is needed and the rate of reaction is

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