The limitations of the experiment are the accuracy of the measurements and the precision of the concentration of gelatin and gum arabic. If this experiment were replicated, an improvement could be measuring the amount of gelatin and gum arabic precisely. Also more than 1 slide could be made with the same concentration, to ensure accurate
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to
The best conditions are cold temperature, high concentration and a high pH.The conditions would be different for different enzymes because all proteins are different. 6. How would you design an experiment to show how much faster H2O2 decomposes in the presence of an enzyme then it does without the enzyme? Use the same system and just add it with water and compare both of them. 7.
Gelatin hydrolysis test is used to detect the ability of an organism to produce gelatinase (proteolytic enzyme) that liquefy gelatin. This process takes place in two sequential reactions. In the first reaction, gelatinase degrade gelatin to polypeptides. Then, the polypeptides are further converted into amino acids. The bacterial cells can then take up these amino acids and use them in their metabolic processes.
Exercise 1 1. Suppose a household product label says it contains sodium hydrogen carbonate (sodium bicarbonate). Using your results from Data Table 1 as a guide, how would you test this material for the presence of sodium bicarbonate? B BoldI ItalicsU Underline Bulleted list Numbered list Superscript Subscript33 Words
Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.
It was also to see how you can identify which biomolecules are present in a substance or a food. I think we achieved the purpose of this experiment and fulfilled what it intended us to do. As we come to the conclusion, I have learned that my hypothesis is indeed correct and there was glucose,starch, and proteins present in the unknown substance. My lab group personally had no idea what the substance was before or after the experiment took place, because glucose, starch, and proteins are present in many other things so learning that these molecules were present didn’t really help us find out what the substance
Proteins were found in the victim’s stomach contents by exposing the contents to the Biuret solution and getting a positive result after the solution became purple. Protein can also be found in the meat of the victims typical, “…pizza with sausage, pepperoni, and bacon…”. Lastly, starches were found in the victim’s stomach contents though exposing the victim’s stomach contents to the Iodine solution and getting a positive result after the solution became a dark color. In pizza, starches can be found in the entire
Bio Chem lab Report 04 Enzyme Biochemistry Group Member: Chan Man Jeun Duncan (16002621) Law Sze Man (16000478) Introduction Enzyme is a protein base structure substance in our body. It works at a biocatalyst that will catalyzing the chemical reaction, which helps to speed up the chemical reaction. Enzyme could only function in specific shape, and the shape of enzyme is depending on the environment, therefore it is hard for an enzyme to function well in an extreme environment. The aim of this experiment is to see can the enzyme functions normally in different environment(pH, temperature and salt concentration) via using starch solution, amylase from saliva, 0.5M HCl solution, 0.5M NaOH solution and NaCl solution, and using iodine solution
The goal of this experiment is to see the anti-cow antibody bind to cow serum only, and we expect to see the anti-cow antibody bind to the spot that had the cow serum. The system we used is the serum from Cow, Horse, Goat, Sheep, and Donkey, Chicken. In order to able to detect and analyze proteins based on their ability to bind to a specific antibody, the SDS-PAGE and Western Blot was performed.
The purpose of this lab is to determine the relationship that exists between the number of amylase gene copies and ancestral diet. As the human civilization moved forward toward agriculture the diets of humans also changed. Depending on where the humans originated would give insight to how much of their diet was starch based. My family’s geographic origins are from China. Thus knowing that the country has a high starch based diet, we would suggest that I would have a high amylase production.
Column chromatography set-up After setting up the column, 2 10-ml of the chosen solvent was obtained and was placed in two separate test tubes. Using a dropper, ~0.5 mL of the food dye was put into the column by dropping it at the side of the column in a circular motion. The chosen solvent was then added just after the green food