The supernatant was assayed for SOD activity by following the inhibition of epinephrine auto-oxidation. 0.5ml of sample was diluted with 0.5 ml of distilled water, to this 0.25 ml ethanol, 0.5 ml of chloroform (all reagents chilled) was added. The mixture was shaken for 1 min and centrifuged at 2000 rpm for 20 min. The enzymatic activity in supernatant was determined. To 0.05 ml of carbonate buffer (0.05 M, pH 10.2) and 0.5 ml of EDTA (0.49 M) was added.
2. Add this solution to the cooled suspension of diazotized sulhanilic acid in the beaker. Stir the mixture vigorously. In a few minutes, a red precipitate of helianthin should form. Keep the mixture cooled in an ice bath for about 15 minutes to ensure completion of the coupling
Metal chelating activity Briefly, 2 mM FeCl2 was added to different concentrations of test sample and reaction was initiated by the addition of 5 mM ferrozine. The mixture was vigorously shaken and left to stand at room temperature for 10 min. Absorbance was measured at 562 nm after 10 min.8 % Inhibition = [(AB - AA)/AB] x 100, where AB, absorption of blank sample, AA, absorption of test sample. 2.6. Antibacterial
The sample was transferred to a 250 ml conical flask kept in water bath for alkali treatment. 75 ml of 17.5% caustic soda was measured using a measuring cylinder at 20°C. 15 ml of 17.5% NaOH was added and fibres were macerated gently with a flattened glass rod for 1 minute. 10 ml more NaOH was added and the solution was mixed for 45 seconds. 10 ml NaOH was again added and mixed for 15 seconds to make lump free slurry.
Decomposition of Aspirin Studied with UV/Visible Absorption Spectroscopy Aims: To determine the concentration of salicylic acid, formed from the hydrolysis of Aspirin, at regular intervals using the UV/Visible Absorption Spectroscopy From the concentration of salicylic acid, concentration of Aspirin to be determined using an equation Calculate the rate constant of this reaction and its order from a plot of graph of ln(aspirin) vs time Discuss the overall flaws and improvements to the experiment Results: As per schedule1, 0.212g of aspirin was added to 50 ml boiling water to form salicylic acid in a 100 ml flask, of which 1 ml was then pipetted to a 50 ml volumetric flask at the 5th min. Following an ice bath, the solution was mixed
Alginate sample (30 mg) was hydrolyzed in 10 mL HCl (0.3 M) at 100 ºC for 2 h. After cooling, the mixture was centrifuged (6000 rpm, 45 min), and the supernatant solution was separated and neutralized with 1 M NaOH and referred to as fraction A. The insoluble material was dissolved in 1 M NaOH and the pH was decreased to 2.85 by the addition of 1 M HCl. The suspension was recentrifuged and the supernatant was separated and referred to as fraction B. The insoluble fraction was dissolved by neutralization with 1 M NaOH and referred to as fraction C. The fractions A, B, and C are enriched in MG, MM, and GG blocks
Assignment 3: Synthesis and Characterisation of L-glutamic acid: Procedure: 1) Approximately 4.40g of L-glutamic acid was added to 0.1L of water. The solute was dissolved completely using a sonic bath. The solution was placed in a thermostatic bath at 80°C and left for 30minutes. 2) After this period of time, the solution was removed and equally distributed into three boiling tubes labelled A,B&C. 3) Then, the boiling tubes were left in a water bath at 45°C.
During this step, I observed that there were bubbles in the solution, especially at the bottom of the beaker. After adding the HLC, there solution had a slight yellow tint. Next, I mixed 0.529g of sodium acetate in 3mL of water and added 0.679g of acetic anhydride to the aniline solution and immediately added sodium acetate. The solution was cooled in an ice bath for fifteen minutes. During this time, I noticed the formation
in the first step benzoic acid was reacted with excess of thionyl chloride using acetonitrile as a solvent and keeping the mixture on an ice bath for 3-4 hours (labeled as reaction mixture a) In the second step gemcitabine hydrochloride along with 3eq tri-ethyl amine and using ethanol again as a solvent was stirred for 15-20 minutes without ice-bath. next with a poisterizing tube the reaction mixture a was drop wise added to reaction mixture b yielding a third and final, reaction mixture c giving off white fumes of socl2. it is stirred for 19 hours and 15minutes at 80c and colour changes to light yellow The preparation of benzoyl chloride from benzoic acid using thionyl chloride at 0’c is an in-situ preparation procedure: FILTERATION: Evaporate reaction mixture and dissolved in hexane and then filter it. The best TLC system for filterate is ethyl acetate : hexane , 4.5:0.5 3.3.2 PROCESS 2 FIGURE 3.5ACETYL DERIVATIVE PROCEDURE In 75mg of gemzar, 2ml ethanol is added and then solubility is checked. After 5 minutes add 0.1 ml (5 drops) DMF, then add 0.104ml Et3N and add 0.036 ml acetyl chloride and stirr it for 17 hours r at 47C
Twenty tablets were weighed accurately and powdered. An amount of the powder equivalent to 5 mg of amoxicillin trihydrate (content of one tablet) was dissolved in 60 ml of diluent. The solution was stirred for 10 min using a magnetic stirrer and filtered into a 100 ml volumetric flask through 0.45µ nylon membrane filter. The residue was washed 3 times with 10 ml of diluent and then the volume was completed to 100 ml with the same solvent. This solution was diluted with diluents to gae a concentration of 0.1 mg/ml solution each of Amoxicillin trihydrate.
1. 150 ml of boiled water was poured into each of the three beakers labeled A, B, C. 2. Five tea bags were soaked for the time given by the manufacturer (two minutes) , in beaker A (Control). The teabags were immediately removed after the time elapsed. 3.
The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.