ipl-logo

Agglutination Test Lab Report

1772 Words8 Pages

ABSTRACT
This paper scrutinises on the importance of agglutination reactions in clinical testing and diagnosis of various diseases. The ability of various antigens and antibodies to agglutinate when mixed in desired environment has been used as the basis to detect the presence of respective antigens in body. It focuses on importance of this method as it gives the results faster than various other methods and provides visible results. Diagnosis of various diseases can be done by this method provided the antibodies are present in blood, urine, plasma or fluid of bone marrow. The following paper discusses the clinical application of agglutination reaction in testing the ABO, Rh blood grouping, test for typhoid …show more content…

The reactions involving such kind of antigen and antibody interaction are known as agglutination reactions. This type of reaction occurs at the surface of erythrocytes, bacterial cells or animal cells at which the antigens are present [1].The results of these reactions are clearly observable since they form large sedimented particles in form of visible clumps. Formation of clumps is due to antigen and antibody complex formation. The ease of its performance and identifiable results make it viable for large clinical importance. Its most important clinical application involves the detection of bacterial infection. This can be tested by simply mixing the serum of suspected individual which contain the antibodies with the antigens of specific bacteria the accumulation of clumps confirms the presence of particular bacterial infection.[2] This test can be performed in various ways including slide agglutination reaction, tube agglutination reaction, indirect agglutination inhibition reactions etc. Another important practical application involves blood group test of …show more content…

Rh antigen is also present on the surface of RBCs similar to A, B and O antigens. Test for Rh blood grouping can be performed easily by side agglutination test. This blood group could be the most complex one of all blood type systems since it involves 45 different antigens on the surface of red cells that are controlled by 2 closely linked genes on chromosome 1.[5] The inheritance of this trait can easily be predicted by knowing the simple genetic concept that the homozygous dominant i.e. DD and heterozygous i.e. Dd are Rh +ve and homozygous recessive i.e. are Rh

Show More
Related

  • Jessica Lunsford Case Study

    1685 Words  | 7 Pages

    The first step is to place a drop of distilled water on a sterile swab and swab a small portion of the suspect substance, The place the swab against a presumptive blood test and an immediate color change should occur if the reaction is positive. If the stain is negative the suspected blood stain may be ignored. If the reaction is positive an evidence number is assigned to the suspected blood and the stain is photographed and measured. The forensic technician should note the stain was collected due to a positive reaction. The blood is then collected and packaged, and the package is sealed and labeled for identification Upon collecting blood if the blood is wet a sterile swab should be rotated in the stain, transferring the blood to the swab.

    Read More

  • Nt1310 Unit 5 Nursing Case Studies

    433 Words  | 2 Pages

    As of 2013, the number of people with MS was estimated to be over 2.3 million worldwide. ( atlas 2013) Most of the non-traumatic disabilities in young adults are caused by MS [1]. It is considered that an interaction of genetic factors, environmental predisposition, and abnormal immune responses can be the chief causes of MS, But the exact etiology of MS is still in question [2]. MS has been greatly studied within the recent years, but a great number of clinical challenges still remain in regard to diagnosis, prognosis, and treatment.[6]

    Read More

  • Essay On Anna Garcia

    1279 Words  | 6 Pages

    If the blood clumped when Serum was added a “+” was placed in the chart, if it did not clump a “-“ went in the chart. Knowing if the blood clumped or not helped determine the person’s blood type. Anna had no “-“ with Anti-A Serum and “+” with Anti-B Serum, we determined that she had B blood as well as Erica Piedmont. When experimenting on the crime scene blood, the team came to a conclusion that it was also B blood. As a result we knew that Anna Garcia and Erica Piedmont have the same blood as the crime scene.

    Read More

  • Miracle Gro Lab Report

    915 Words  | 4 Pages

    The data observed and recorded in this lab shows that the concentration of miracle gro’ does affect the growth rate and germination speed of black eyed peas. The data is shown through two graphs and two data tables. The control group in this experiment is the seeds with a 0% concentration of miracle gro’, therefore the seeds with just water. The experimental groups are different concentrations of miracle gro’ including a 10%, 15%, 20%, 25%, and 30% concentration. The variable in this experiment is the amount/concentration of miracle gro’.

    Read More

  • Staphylococcus Epidermidis Essay

    771 Words  | 4 Pages

    Staphylococcus epidermidis is the organism that was identified based on the tests that I had conducted. The tests that I used to identify this organism were the coagulase test and the catalase test. My bacterium was beta hemolytic as well. First, a gram stain had to be done to determine whether the organism was a gram positive organism or a gram negative organism. This determined which set of tests that had to be done.

    Read More

  • Unknown Microbiology Essay

    517 Words  | 3 Pages

    Catalase activity test establishes whether the bacterium produces the enzyme catalase. The eosin methylene blue test or EMB, inhibits the growth of gram positive bacteria and tests whether or not gram negative bacteria can ferment lactose. Lactose fermentation testing is done to see if the bacterium is capable of fermenting sugar by testing for acid and gas production. These are the possible tests that are needed in order to identify unknown

    Read More

  • K & U5 Unit 5 Diagnosis Of Malaria

    904 Words  | 4 Pages

    5. Serology Serology antibodies identifies against malaria parasites, using either indirect immunofluorescence (IFA) or enzyme-linked immunosorbent assay (ELISA). Serology does not detect present infection but rather measures precedent contact. 6. Drug Resistance Tests

    Read More

  • Serum 2216 Lab Report

    592 Words  | 3 Pages

    The addition signs for each test on serum 2216 illiterates how concentrated the molecule is like purple for example was the most concentrated due to the amount of addition signs in the table. For Macromolecule test 1 and 2 the sample was diluted. Both the concentration and decreased by an equal set of intervals. The volume of distilled water is what was added and the results illustrate how deeply colored each concentration was. Each cup was diluted with the test pertaining to it with a syringe that explain the volume and the concentration is from heating or mixing the sample throughout the investigation.

    Read More

  • Drug Testing In The Workplace Essay

    811 Words  | 4 Pages

    One of the most prevalent method being Saliva Drug Test. Saliva provides a non-invasive and quick specimen for drug testing. A Saliva Drug Test ranges from detection of alcohol to cannabis, HIV antibodies to steroids. Saliva Drug Test enables a screening in a very short detection time period. They are designed to tell if someone has recently used a drug.

    Read More

  • Seasonal Allergy Research Paper

    579 Words  | 3 Pages

    Seasonal Allergies Everyone has listen to the term "allergy", and we know what exactly is it , and how it begins, but now we need to learn how doctors diagnose seasonal allergies? and know what cause its symptoms? To answer these questions, we have to refer to the processes that doctors follow to diagnose seasonal allergies . Doctors first ask some questions about the kinds of symptoms, family history with seasonal allergy, the period of the season that appear on, if the patient has any bets, other medical problems, if the patient smoke , and other questions .

    Read More

  • Essay On Blood Count

    711 Words  | 3 Pages

    Performing the complete blood count gives us the exact amount of hemoglobin in the patient’s blood. The hematocrit, which is defined as the fraction of blood composed of red blood cells, is also able to be examined by performing the complete blood count test. Although the above mentioned findings are the most common use of this test, this test also allows us to gain information on the measurements of the average red blood

    Read More

  • Organ Bath Experiment

    1445 Words  | 6 Pages

    Next, a basic stock solution was used to prepare various concentrations ranging from 1.0 x 10-8M to 1.0 x 10-1M by serial dilution. The tissue was washed by overflow with reservoir’s solution for 5 seconds to obtain baseline before adding 0.1ml, 0.3ml and 0.5ml for each concentration respectively into the tissue bath. The tissue’s peak response for each final bath concentration(FBC) was measured and recorded. Rmax and EC50 of histamine were recorded.

    Read More

  • Arginase Lab Report

    1437 Words  | 6 Pages

    The reaction that occurs can be investigated via the adding of the liver extract which contains the arginase to urea concentrations and distilled water. The amount of urea formed is determined via spectrophotometric analysis α-INPP. The urea produced was known via the color reaction with the α-INPP, it is the reagent used for the colorimetric determination of urea. (Barry J, et al. 1984). The red color formed when the α-INPP is reacted with the urea, is sensitive to light thus it is photo labile.

    Read More

  • Why Are Blood Groups Important

    1733 Words  | 7 Pages

    The Rh blood group system is one of the 35 known human blood group systems which consists of 50 defined blood group antigens,

    Read More

  • Thalassemia Case Study

    3218 Words  | 13 Pages

    It is a genetic disorder that is inherited from both parents who are carriers of the disease. It is characterized by anaemia with small and pale red blood cells (hypochromic microcytic anaemia) due to reduced production of one or more globin chains in the red blood cells. The consequences are ruptured of red blood cells in the spleen (Fucharoen, 1987; Weatherall, 1997). The clinical severity of thalassemia varies greatly depending on the number of genes affected (Cornelis, 2010). Approximately 7.0% of the world’s population is thalassaemia carrier and an estimated 300,000 babies are born each year with this disorder.

    Read More

More about Agglutination Test Lab Report

Open Document