# Importance Of Spectrophotometry

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Spectrophotometry is actually defined as the quantitative computation of the transmission and reflection of properties of a material in terms of the wavelength  and it involves the use of a spectrophotometer. A spectrophotometer is a standard research tool that can be utilized in various fields worldwide and it is basically consists of a spectrometer and a photometer that can produce and determine monochromatic light transmitted or absorbed by a liquid sample in order to identify the material. Furthermore, the bandwidth of the spectrum and the measurement of linear range of absorption or reflectance are also stated as per important features of spectrophotometer.  The spectrophotometer was invented by Arnold J. Beckman and his colleagues…show more content…
It is a useful and applicable for determining the concentration and the absorbance of an unknown sample. The equation of Beer’s Law can be shown as follow:
Beer’s law: A = k • C A=Absorbance of the sample k = constant C= concentration of the sample As far as the equation is concerned, the absorbance of the sample is directly proportional to the concentration of the sample. This means that the more concentrated the solution of the sample is, the greater the absorption by the sample will be. The k value is dependent on the wavelength of the light used, the nature of the solvent and the travelling distance of the light. Since the value for K is always varying, therefore, it will be kept in constant to ease the burden of measuring the concentration sample. However, the absorbance of the solution cannot be measured directly. Therefore, transmittance is introduced into equation.
A = log (1/T) = -log (T)
A=Absorbance of the sample
T= Transmittance of the