The second time you go through, change out the test tubes as soon as you get to your first plateau, this liquid is liquid one. Wait until the temperature gets to your second plateau and change it out again, this liquid is the junk liquid, you don’t need it. Now wait until it is all done and the final liquid is your liquid two. Liquid one and Liquid two are now your separated two liquids and you can test the
Oxidase reagent 8. TDA reagent Procedure • Preparation of a 0.85% NaCl solution I. Add 0.85% of NaCl solution into a bottle with sterile distilled water to form a 5 ml of solution and mix well in a McCartney bottle. • Preparation of the bacterial suspension I. Flame an inoculation loop until red hot in the blue flame of the Bunsen burner and allow it to cool it few seconds. II.
We then take the sample out of the water and immediately wipe it thoroughly, after which it is placed in the calorimeter with tap water. We then stir the water around the sample in order to quickly attain thermal equilibrium. We record the final temperature once thermal equilibrium of water and the sample together
Ensured the complete dissolution of Agarose by checking against the light. 3. Cooled the solution to 50°C and added 4.5 l of ethidium bromide dye to a final concentration of 0.5g/ml and mixed thoroughly. 4. Slowly poured the warm Agarose solution into the casting tray and inserted the required combs.
1- Extraction method No. 1: Fifty grams of powdered aerial parts of portulaca oleracea were hydrolyzed by using reflux for 9 hr. with 300 ml of 2N hydrochloric acid then the extract cool at room temperature ,filter and wash the residue with 2N of ammonia solution. The residue dried overnight at 60ºC ,the final step involve the extraction of the residue with 250 ml of chloroform by using soxhlet ,the final extract cool at room temperature ,then filter and evaporate to dryness by using rotatory evaporator at 40ºC to yield (2.264 gm),as show in scheme (2-1). 184.108.40.206-Extraction method No.2: Fifty grams of powdered aerial parts of portulaca oleracea extracted by soxhlet with 500ml 0f 70% ethanol for 8 hr.
Because of inhabitation property of water toward complete reaction, the methanol and water mixture is separated from the oil phase. Afterward, further methanol and acid catalyst can be added and the reaction continued for the next step. We should note that if the number of pretreatment steps be increased, the ester yield reduces owing to the solubility of ester in methanol . For that reason, in this study, we tried to reduce the FFA level through one step pretreatment to achievement high ester yield and also save the time for producing BD. After each esterification, total WCF was washed twice with hot and distilled water to eliminate any reminded acid in
Prepare a wet mount slide of the rhubarb tissue in distilled water only. View your slide under low power on your microscope, and then switch to high power. Draw a diagram of the field of view, and label. Irrigate your slide with the salt solution. Leave for a few minutes.
Each electrode was placed in its correspondent solution (Note: if the metals’ places were accidentally changed, some defilement can occur. In this case, renew the salt bridge, use new solutions and clean the metals again). In order to make a salt bridge, a streak of filter paper was moistened in the beaker with saturated potassium nitrate. In the next step, the ends of a paper were placed in each electrolyte. It should not touch the metals.
(3) Rusto-cam (acidic) The rusto-cam used for maintain the pH of the liquid. (4) Solvo clean (acidic) Also the solvo clean is used for the removal of oil and grease. Then the part is again sent for water cleaning known as water rinsing. And then forwarded for the iron-phosphating. 3.1 (c) pre-treatment process – iron-phosphating The layer of the iron- phosphating is applied on the degreased metal surface.
The PVC pipe is fixed onto the caulk gun with the washer fitted at the opening end so to prevent the briquettes from flowing out when pressed. When ready, the clamp was screwed onto the pipe and the gun was pressed to apply pressure to form the biomass briquettes. The biomass briquettes were then taken out to be sun dried for 3 days (Fig. 3). This procedure is then repeated for all the briquettes with combination with varying