Once 3-4 readings for the solution were collected, the 2M NaOH was added to the solution. The lid was quickly replaced in order to prevent heat from escaping and not being recorded by the temperature probe. The cup was swirled until the temperature reached a peak and began decreasing. After the 180s had passed, data collection ended. The solution was discarded into the waste bin, and the materials were washed.
These bubbles are made because the reaction is causing carbon dioxide to be released. For a good portion of the shell to be removed, you will have to wait approximately 12-24 hours. You will know when you are making good progress when there is a white frothy scummy layer on the top of the vinegar. Next, you will be able to remove the egg after a day of being soaked in the vinegar. You may want to pour the vinegar into another cup and catch the egg in your hand.
Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome. Lastly, a glucose fermentation test was conducted to determine the unknown organism.
Prepare a wet mount slide of the rhubarb tissue in distilled water only. View your slide under low power on your microscope, and then switch to high power. Draw a diagram of the field of view, and label. Irrigate your slide with the salt solution. Leave for a few minutes.
Shake funnel and rinse off water layer ( This contains the sulfuric acid and majority of methanol). Again wash the ether with 25ml of water and then wash the organic layer with 25ml of 10% sodium bicarbonate to extract unreacted benzoic acid. Again shake separatory funnel with frequent venting of pressure and opening the stopcock. Allow the separation of layers and drain off bicarbonate layer into a beaker. Wash ether layer with saturated sodium chloride solution and retain ether layer.
TLC profiling Ammonia in butanol was the appropriate solvent to use for the column chromatography of food dye. After testing for the appropriate solvent, the set- up for column chromatography was done (Figure 2.). With the use of a clamp, the column was clamped onto an iron stand. A small cotton ball was then pushed in the column until it reached the bottom by using a stirring rod. A small amount of sand was added after the layer of cotton.
Compare the result to the chart on the back of the urinary pH test strips bottle, and record data. Clean the stirring rod with water before moving on to the next test tube. Repeat this process for each increment (2 mL, 3mL, 4mL) Figure #1: Picture of bean solution mixed Figure #2: Picture of materials needed for the with alpha galactosidase experiment Safety considerations: Be careful with the beakers, glass stirring rod, and test tubes, as they could break easily and can cause cuts in the skin. DCP: A scatter plot will be used to display how the amount of alpha galactosidase (measured in mL) in the bean solution affects the glucose concentration (measured in mg/dL) and error bars to show the standard deviation. A line of best fit will be used to show the relationship between the glucose concentration and the amount of alpha galactosidase.
Ignite with a meker burner for about 1 hour. Complete the Ignition by keeping in a muffle furnace at 500 °C to 570 °C until grey ash results. Cool and filter through whatman filter paper No. 42 or its equivalent. Wash the residue with hot water until the washings are free from chlorides as tested with silver nitrate solution and return the filter paper and residue to the dish.
Eosine and Methylene Blue EMB The test for Unknown 361 showed a pink color, which signified that the fermentation occurred very slowly and poorly. Procedure: 1. Obtain an EMB agar plate 2. Streak it with the appropriate bacterial culture using the quadrant streak plate method 3. This will result in the isolation of individual colonies.
Glass plates were filled ¾ and the gel was covered with 100-500 µl Isopropanol in order to achieve an even surface. When the gel was polymerized, isopropanol was removed on the top of the running gel with water and thus, water was also removed as well. Then, stacking gel was prepared and its polymerization reaction was started with TEMED, and its solution was quickly poured on the top of the running gel. Therefore, a comb was inserted into the stacking gel before its polymerization, to form the loading wells. When the gel was polymerized, the inner chamber was filled with electrophoresis running buffer and the outer chamber was filled half.
sides. Every type of bacteria has a different morphology, it is important to distinguish it to aide in identifying bacteria. The last test that should be performed after reviewing the results of the streak plate is the Catalase test. This test is used to see if the bacteria produces catalase, which is an enzyme that breaks down hydrogen peroxide (H2O2) into H20 and O2. If this test is positive, the hydrogen peroxide which is dropped onto the colonies in the streak plate will begin to bubble.
Research Protocol – Monitoring of the Daphnia magna heart rate The experiments focused on the four treatments of nicotine, caffeine, ethanol, and double distilled water (placebo). 180 μL of each bioactive solution (nicotine was covered with foil, due to light sensitivity) and 120 μL of double distilled water were placed into labeled eppendorf tubes to dismiss cross-contamination, and were placed on ice to match the environment of the Daphnia to reduce any added stress on the Daphnia. One Daphnia was placed on the testing petri dish, and then all the excess pond water was removed with a transfer pipette. Immediately 10 μL of double distilled water was added with a micropipette; this way our concentration of the treatment was the intended concentration.
Methanol was filled in a test tube and placed into a water bath to heat up. 12 Drops of the Methanol were then added to each flask until the crude caffeine had completely dissolved. 13. The solution was then filtered and the residue collected in a filter paper. It was left to dry and
This procedure occurred in the presence a Bunsen burner. The inoculation needle were placed within the open flame 15-20 second in order to sterilize the needle and prevent contamination. The needle was allowed to cool 5-10 second before inoculation. Using aseptic transfer technique the needle was used to gather up some of the colonies on the plate, making sure not to touch anything else with the needle. The test tube was uncapped and the lips of the test tube was passed through the open flame three times.