Eimeria Oocyst

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Collection and Prepration of the Inoculum The fecal matter was collected from naturally infected sheep and it was examined for Eimeria oocysts by a coverglass flotation method using Sheather's sugar solution to concentrate the oocysts (Sloss and Kemp, 1978.). The infected fecal material was mixed in 2.5 % potassium dichromate solution and was left for sporulation at room temperature for one week. The sporulated oocysts were subsequently quantified using the Mc master technique (Maff, 1986). A strict morphological criterion was used to specify Eimeria species present depending on the morphometric character according to Levine and Ivens (1986). Experimental Animals Thirty healthy lambs aged from 4-6 months, free …show more content…

One hundered ml of representative sample of rumen liquor was brought immediately to the laboratory in a closed container tightened with a rubber stopper to sustain anaerobic condition during transport. Samples of rumen liquor were sieved between two layers of gauze to remove debris. Two 5ml duplicate liquor were separately taken and diluted five times by saline solution and lugol's iodine to fix and stain protozoan cell. 0.1ml of the diluted ruminal fluid was poured on a clean glass slide which was then covered by a clean cover slide. The total protozoa count / 1ml = average count in 30 field X 1173 (area of the cover slide X 50). Each of the two duplicates was counted and the average was taken (Abou El-Naga 1967). Evaluation of the viability of the ruminal protozoa was done by counting the proportion of motile ciliates under microscope (Nsabimana et al. …show more content…

The examined blood parameters were total leucocytic count (WBCs), lymphocytes count , monocytes count, granulocytes count, total erthrocytic count (RBCs), hemoglobin concentration (Hb), haematocrit concentration (HCT) and mean corpuscular hemoglobin concentration (MCHC). The reference normal level of the blood parameters of sheep was in accordance to Radostits et al.

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