Background Information
Nowadays, it is common to find people around us are suffering with the lactose intolerance; babies are able to digest lactose because they can produce lactase while many adults are suffering the inability to digest lactose. As the research illustrates: “when lactose moves through the large intestine without being properly digested, it can cause uncomfortable symptoms such as gas, belly pain, and bloating” (Lactase enzyme lab, 2017), so the effects from lactose intolerance lead to the need for the enzyme to add into the milk. In order to let the people with lactose intolerance can still drink the milk in their daily lives, some milk companies develop a special kind of milk which is added with the lactase and this milk
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The glucose concentration in the regular milk sample is measured by using the test strip.
The directions that came with the glucose test strips are followed; a glucose test strip is dipped into the milk sample.
The time is counted down for 30 seconds in terms of the strip directions.
The color of the strip is compared after dipping it in the milk with the color-coded key on the side of the bottle to determine the concentration of glucose in the milk.
The glucose concentration in the lactose-free milk sample is measured by using the test strip.
The results are recorded in the lab notebook in the same section as the experiment of glucose and water.
One drop of the lactase solution is added into the regular milk sample which is the one contains the lactose.
The milk is warmed by conveying the heat from the hands for 2 minutes.
The glucose test is repeated by using a new test strip.
The result is recorded in the prepared lab notebook.
One drop of the lactase solution is added into the lactose-free milk sample.
The milk is warmed by rolling the cup back and forth in hands for 2
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Based on the data which was collected, the glucose concentration of regular milk was 0-2.8mmol/L while the glucose concentration of the lactose-free milk was 14.0-28.0mmol/L. The data clearly showed that a higher lactose concentration was contained in the lactose-free milk compared to the glucose concentration of the regular milk. After the enzyme lactase was added into the lactose-free milk and the regular milk, the glucose concentration was measured again by using the glucose strips. The glucose concentration of the regular milk increased: the glucose concentration of the regular milk became 5.6-14.0mmol/L because of the lactose. Besides, the glucose concentration of the lactose-free milk with the lactase was close to the glucose concentration without adding the lactase, but not exactly the same. If we were able to do our experiment again, we would prepare more urinalysis strips so that we could do the experiments for more than one time which could reduce the inaccuracy of the
Without question whole cow’s milk is high in saturated fat and cholesterol, both of which should be avoided. In addition, there has been growing concern that three health issues associated with milk consumption have gone ignored and demand immediate attention: lactose intolerance, type-1 diabetes mellitus, and prostate cancer. A study in 2002 examined “the links between milk consumption and both type 1 diabetes mellitus, and lactose intolerance. These 2 topics...have received major and extended coverage in the media and have raised broad concerns about the safety of milk for children” (Goldberg, Folta, Must). In this study were revealed results of a Finnish study of cow’s milk consumption where the authors stated “...these data support the hypothesis that there may be a subset of at-risk children for whom cow's milk consumption promotes the development of type 1 diabetes” (828).
According to "Got Milk ? You Don 't Need It," Mark Bittman (2012) suggests that "propaganda" is being stimulate by the American government despite the fact many people have sensitivity toward milk, and there is other alternative to milk. The writer focuses on the reasons why people do not have to drink milk. First, he is explaining that milk allergy is one of the most common allergies in United of States, and that many people are suffering from stomach problems. Moreover, he provides his experience with cutting dairy output to cure his stomach issues
When given an unknown bacteria there are a multitude of steps one must go through to be able to correctly identify what bacteria was given. It is important to correctly identify the bacteria because some bacteria are more harmful than others. The gram stain is the first test that should be performed because it helps narrow down the possibilities by telling one whether the bacteria is gram positive or gram negative. After this test is performed, one shall place bacteria on/in Mannitol Salt agar, MacConkey agar, Eosin Methylene Blue agar, Urea agar, Simmon’s Citrate, Purple Beef broth with Lactose and finally Purple Beef broth with Sucrose. A streak plate should also be made up, this helps one identify the morphology of the colonies.
It is necessary to understand what each test reveals about the unknown. Citrate tests are performed in order to distinguish between different enteric bacteria by seeing which can use citrate as the sole carbon source. MR/VP are tests that are used to distinguish between different types of fermentation either mixed acid or butanediol and test for the production of acetoin. H2S production is used to determine whether or not the bacteria can produce hydrogen sulfide. Mannitol high salt testing is done in order to determine if the bacteria is salt tolerant and can ferment mannitol.
We will provide free glucose meters and strip to the individuals with diabetes to be able to measure their A1C level. We intend that this measurement will be done every two to three month to be able to determine their average blood level. According to the America diabetes association, the A1C level of diabetes patient is good to measure because it helps professionals to know how oxygen flow into the blood cells and also help to indicate the amount of sugar stick in the red blood cell (RBC). The goal for this measurement is help us know how well the patients are able to manage their diabetes by healthy
If there is a color change, then it is known that protein is present in the solution. Finally, lipids are tested. 5 mL of water are added to 5 mL of oil. 5 drops of Sudan 3 are added, and if the color changes, then lipids are present. Next, the McMush is tested.
In a non-reducing sugar 3cm cubed and 10 drops of hydrochloric acid is placed in a test tube for a water bath of 5 minutes to be mixing afterwards. Biurets reagent is added to the protein solution to determine it presence. Testing for
The Study of Diffusion and Osmosis Using Deshelled Eggs Maquita A. Dieufene Jessica Thelwell(Partner) 10/09/2014 1611 Evening Lab Introduction It is quite simple to overlook the roles diffusion and osmosis play in daily life. If one has ever spent too much time in the pool and watched as their fingers begin to turn prune-like, that is an example of osmosis. Osmosis is simply defined as the movement of a concentrated solvent through a semi permeable membrane to a more concentrated solvent.(Biology Corner) Relating to the earlier example of osmosis, your body acts as the more concentrated solvent for the water to penetrate. Diffusion is the exact opposite of osmosis.
Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
C12H22O11 (lactose) + H2O > 4CH3CHOHCOOH (lactic acid) is the equation for lactose to lactic acid.
B-galactosidase breaks down the disaccharide lactose into simple sugars glucose and galactose. However, glucose is a colorless compound hence it has to be substituted with a compound that is detectable by a visible color change. Hence,
Lactose intolerance, also known as milk intolerance, is the inability to fully digest the sugar, lactose, in milk. There are more than three million cases in the U.S. per year. As a result, they have diarrhea, gas and bloating after eating or drinking dairy products. A deficiency of lactase, an enzyme produced in your small intestine, is usually responsible for lactose intolerance. Many people claim to be lactose intolerant.
5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).
Place the the beaker onto a hot plate that is on a low heat setting (about setting 3). Every 5 minutes for 20 minutes, measure the circumference of the balloon and record it in Data Table A. You can measure the circumference of the balloon by looping a piece of string around it then using a ruler to measure the string’s length. Record the data in the data
Then, the boiling tubes were left in a water bath at 45°C. After 5minutes, the boiling tub, labelled A, was withdrawn from the water bath. 4) The sample was filtered using a Buchner funnel, collected in a sample vile, labelled A, and positioned securely to dry and crystallise in an oven at 65°C. 5) After 45minutes, the boiling tube, labelled