In order to synthesize this compound, measure out 1 gram of NaC2H3O2.Weighed 1 gram of NaC2H3O2 and mixed it with ionized water. Boiled 12 mL of 1.0M Acetic Acid added into a beaker containing the sodium carbonate on a hot plate until all the liquid is evaporated leaving solid precipitate form inside of the beaker. Weigh the precipitate form and mix it with water until fully dissolved. Weigh the precipitate remaining. A flame test and a halide ion test were performed.
Eventually using the NaOH and the acid’s consumed moles, the equivalent mass will be determined. Procedure: Part 2: Obtain 45mL of NaOH, and then weigh 0.3-0.4g of the unknown acid (KH2PO4). Dissolve the acid into 20.00mL water. Record the buret readings, and slowly titrate the NaOH into
Higuchi model94,95 [Q = KH t½] 4. Korsmeyers-Peppas model:96,97 F = (Mt/M) = Km tn 6.4.7. Drug content: The drug content from pellet formulations was investigated; in which the quantity of pellets equivalent to 6.25 mg of dose of zolpidem tartarate was weighed and dissolved in 0.01 N HCl, sonicated for 15 minutes to dissolve it completely and then the solution of 14 ppm was prepared which was considered as a working level for the complete analysis. The absorbance was determined at the 294.5nm by UV spectrophotometer. Then the drug content was determined by comparing the absorbance of this solution with standard solution having same concentration.
Cool the apparatus to room temperature after the refluxing period. Wash down the interior of the condenser and flask twice with approximately 25 ml portions of distilled water. 9. Remove flask from the condenser and dilute to a final volume of approximately 350ml with distilled
Each 0.1M Sodium hydroxide solution that had been rinsed was drain into the waste container located under the hood. 3. The burette was filled with 0.1M Sodium hydroxide solution(prepared prior of this experiment) to 50 ml volume and the burette was clamp vertically(the air bubbles was remove from the tip of the burette by draining the 0.1M Sodium hydroxide into smaller beaker) 4. The 10g or 10ml amount of samples was inserted into 250ml conical flask and with addition of 50ml distilled water 5. Three to five drops of phenolphthalein were added into conical flask 6.
Throughout the mixing process, the clear red solution slowly changes to a denser red solution (Appendix figure 23). A thermometer was used for temperature checking. The beaker was removed from the hot plate when the temperature was found to be higher than 50 ℃. This was done to prevent a sudden gelation happen before all the active dissolved in the ethylene glycol. Moderate heating of the solution for a period of time is allowed to obtain a wet gel (Appendix figure 24).
Four drops of 0.5 M NH3 solution was added into test tube 5. Four drops of 4 M NH3 solution was added into test tube 6. Your observation was recorded in the table on the data sheet. 20. The unknown metal ion was identified, from the observation of the reactions of the known and unknown metal ions.
One millilitreer of the lichen extract (1 mg/mL) in a volumetric flask was diluted with distilled water (46 mL), and the content was mixed in a volumetric flask after adding. oOne millilitreer of Folin-Ciocalteu reagent was added and the content of the flask was mixed. thoroughly . After 3 min, 3 mL of 2% sodium carbonate (2 %) was added and then was allowed to standleft for 2 h with intermittent shaking. The reaction absorbance of prepared mixture absorbance was measured at 760 nm in a spectrophotometer (Jenway UK).
The dependent variable is the product of the chemical reaction and the formation of gas filling up the bottle eventually causing an explosion. The independent variable is the baking soda and vinegar react. My control conditions are a water bottle that is about 8 inches in height and about 3 inches in width and I will use about 6 grams of baking soda and ½ a cup of vinegar. I will put protective glasses to avoid damage to my eyes and I will first pour the vinegar
Effect of pH To check the pH stability of the bacteriocins, 5 ml aliquots of CFS was taken in sterile tubes and were adjusted to pH values of 2, 4, 7 and 9 busing 1N HCl or 1 N NaOH. After incubation at room temperature for 30 minutes, pH in each tube was readjusted to 6.5 and the antimicrobial activity was determined by agar well diffusion method17. 6.3. Effect of proteolytic enzyme 5 ml of CFS was taken in a test tube and was treated with trypsin-chymotrypsin enzyme (10AU/ml) at pH 7. The test tubes with and without enzyme (control) were incubated at 370C for 1 hour.