Ir Spectrophotometer Lab Report
Working of IR spectrophotometer- Incident radiation is split by instrument in two beam which are energetically and optical identical. One beam pass through sample and other through reference cell. When sample cell contain the sample half beam travelling through it undergoes a decrease in intensity. The two half beam are recombined, they produce an single which is measured by detector. The detector passed the single to recorder through a servomotor which give an output.
APPLICATION OF IR SPECTROPHOTOMETER
1. Determination of impurity
2. Shape and symmetry of molecule
3. In industry, IR spectroscopy used to determine impurity of raw material, quality control and identification of material.
4. Examination of old paintings and artifacts.
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One beam pass through sample and other through reference cell. When sample cell contain the sample half beam travelling through it undergoes a decrease in intensity. The two half beam are recombined, they produce an single which is measured by detector. The detector passed the single to recorder through a servomotor which give an output.
APPLICATION OF IR SPECTROPHOTOMETER
1. Determination of impurity
2. Shape and symmetry of molecule
3. In industry, IR spectroscopy used to determine impurity of raw material, quality control and identification of material.
4. Examination of old paintings and artifacts.
5. It can used to identify the paints on appliances and automobile without destroying the surface.
6. Determination of molecular structure and formation of chelate.
Polarimeter
Polarimeter is a instrument used for measuring the angle of rotation of plane polarized light.
When a certain compound placed in the path of plane polarized light, the plane of polarization is rotated. The property by which plane of polarization of light rotatedis called optical activity and compound posses this property called optically active. The rotator power of given solution is expressed in specific
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