Iron phostate, also extensively known as FePO4, was studied through a special type of transformation process known as neutron powder diffraction. The transformation process uses a temperature that ranges from 297K to 1073K. As long as it is within the low temperature range, it is defined as the ‘α’ phase. Otherwise, if it is within the high temperature range, it is defined as the ‘β’ phase. The iron phostate will change its tetrahedral form in the low temperature range to its octahedral form in high temperature range.
The integration of nanomaterials with biology has led to the development of diagnostic devices, contrast agents, analytical tools, physical therapy applications, molecular sensors and drug delivery vehicles. From all nanomaterials with antibacterial properties, metallic nanoparticles provide the best results. Several types of NPs, including various Molecules of 30 metal and metal oxides, have been developed and evaluated by different research groups; examples include silver (Ag), gold (Au), Ag oxide (Ag2O), zinc oxide (ZnO), titanium dioxide (TiO2), calcium oxide (CaO), copper oxide (CuO), magnesium oxide (MgO), and silicon dioxide
The secondary antibody would the overlap the primary antibody by binding to it. The secondary antibody (anti-rabbit HRP labelled) used is conjugated with an enzyme Horse radish peroxidase which then binds to its substrate tetramethyl-benzidine (TMB) to produce a blue color indicating the presence of lysozyme. SDS-PAGE gel electrophoresis is a process which separates proteins based on molecular weight. The purpose of this method is to separate out the lysozyme by its weight. The weight is known to be 14.6Da.
The first affinity tag was described in 1983 (Mathias Uhlén, 2008). It separates the proteins from endotoxins basing on the reversible interactions between the proteins and a matrix coupled ligand. It delivers excellent selectivity and resolution, provided that a ligand is available that can bind onto to the material of interest. It is believed that either the electrostatic or hydrophobic interactions are a result of the interactions between the specific ligand and the protein of interest. Desorption can be achieved by introducing a competitive ligand, by varying the pH or by changing the polarity of the matrix (GE Healthcare).
To check the anti-aggregation property of Thymoquinone on diverse proteins (2). Project summary There are several evidences that Thymoquinone is a potent inhibitor of amyloid beta peptide (Alhebshi AH, 2013) but there is no evidence that it can also be a potent inhibitor of diverse class of standard proteins. So, we will check the effect of this compound on the aggregation behavior of standard proteins such as Bovine Serum Albumin, Human Serum Albumin and other mammalian serum albumins proteins. Till now we know that these standard proteins and some amyloidogenic proteins such as amyloid beta peptide, tau protein, IAPP, TRR protein are responsible for the diverse diseases which are caused by the aggregation of proteins and amyloid formation
Investigating the Concentration of Iron (III) Thiocyanate Research Question: To determine the optimal effect (equilibrium condition) of manipulating the concentration of both reactants on the values of absorbance detected by a spectrophotometer at 540 nanometers, in between the forward reaction of Iron (III) Chloride (FeCl3) and Potassium Thiocyanate (KSCN). Background Information: I’m an IASAS theatre tech alternate and my main role is being the assistant stage manager, which puts me in charge of handling props as well. It was quite engaging for me to find a reaction that created a chemical replica of hemoglobin; especially after our recent production: The Complete Works of Shakespeare. Thus, it was interesting to find an equal solution
IONIC EXCHANGE CHROMATOGRAPHY This is a form of affinity chromatography that allows for the separation of product and contaminants through the use of specific electrical charges produced by the individual molecules. Resins are applied to depending on the compound one wishes to remove ie if your compound is negatively or positively charged. This system is specifically used after one of the three previously stated methods of separation and an ultrafiltration/diafiltrtion step as it is costly and therefore is not viable for use with large volumes of compound. It is mainly used for the removal of any remaining components from the media and including cell fragments, chemical agents and lipid components. (Duong et al 2014) Ionic exchange resin use
Background Icariin (ICA) is an active compound extracted from Epimedium brevicornum Maxim. Icariin has been reported to have a clinically significant therapeutic effect on rheumatoid arthritis. However, the mechanism by which icariin prevents cartilage and bone degradation has been less explored. Methods New Zealand rabbits were immunized with antigen-induced arthritis (AIA) and treated with icariin for 3 months. Arthritis cartilage from rabbits were prepared for histological analysis, distal femur were scanned using micro-CT. Chondrocytes were observed using transmission electron microscopy (TEM).