Ftir Spectra Of Itraconazole Analysis

PREFORMULATION STUDIES
Standard calibration curve In the pre-formulation studies, the λ max of Itraconazole by spectrophotometric method in phosphate buffer pH 6.8 was found to be 262nm.

Table no. 1.7: Calibration Curve of Itraconazole in Phosphate Buffer pH6.8
S.no. Concentration
(µg/ml) Absorbance
1 0 0
2 2 0.12
3 4 0.26
4 6 0.38
5 8 0.44
6 10 0.57

Fig 1 : Standard Graph of Itraconazole in Phosphate Buffer pH6.8 Drug excipient compatibility study Fig 2: FTIR spectra of Itraconazole Fig 3: FTIR spectra of Itraconazole final formulation

CHARACTERIZATION OF TRANSFERSOMES
EVALUATION OF TRANSFERSOMAL GEL
Vesicle shape and type:
The surface morphology was studied by Optical Microscopy. The shapes of most of

7: Zero order plot for optimized formulation

Fig No 8: First order plot for optimized formulation

Fig No 9: Higuchi plot for optimized formulation

Fig No 10: Peppas plot for optimized formulation

STABILITY STUDIES
The stability studies were carried out according to the procedure described in the previous section. The results were shown in the table below:

Table 2.1: % Entrapment efficiency and % Drug content after stability studies (T6)

Number of Days % Entrapment Efficiency at temperatures % Drug Content at temperatures 4±2oC 25±2oC 37±2oC 4±2oC 25±2oC 37±2oC
15 88.5 87.82 88.83 93.6 93.1 93.6
30 87.3 86.71 88.05 93.1 92.5 92.6
90 87.0 86.18 87.70 92.6 92.7 91.2

SUMMARY The work was carried out to prepare Itraconazole transfersomal gel to achieve controlled release effect at the site of administration
 The pre-formulation studies like UV analysis of Itraconazole, FTIR were complied with BP standards. The FTIR spectra revealed that there were no interactions between the drug and the carriers.
 Transfersome formulations were prepared by thin film hydration technique and were incorporated into 2% carbopol gel. The Formulation T6 containing Lecithin: Span-80 in ratio 85:15(%w/w) has higher entrapment efficiency and maximum drug