Spray the chilli plants in their fourth leaf growth stage with spore suspension containing 3 × 105 conidia ml−1 (15 ml/pot). Spray the non inoculated plants with distilled water containing same amount of Tween 20 (0.02 %). After spraying, keep both inoculated and non inoculated plants in a moist chamber with 98−100% relative humidity for 24 hours. Seven days after inoculation, evaluation of the wilt disease severity is based on visual assessment of lesions caused R. solani on a nine grade scale according to IRRI standards (2002). Disease severity for each replication was calculated according to the equation below (Cai et al.,
Animals: Take male albino rat 220-250 gram having age at least 2months old. Place them in animal house and supply it pellet diet and tap water. Animals are grouped in a number four. Each of the group contains 8 rats. Diabetes induced by injecting streptozocin bt introperitonial route of dose 60mg/kg .
Three months later, the bone mineral density (BMD) of the femurs of all the rats were determined using GE Lunar Prodigy (GE Healthcare Bio Sciences, Pittsburgh, USA) to evaluate whether the model had been successfully established. Once the model is successful, the OVX rats were randomly divided into five groups of 14 animals: OVX group (double distilled water (0.2 mL/100 g, daily, administered orally)), positive group (70 mg/kg, weekly, administered orally) and OVX–HE groups (0.11, 0.33 and 0.99 g/kg, daily, administered orally) and treated for 12 weeks. The 0.11, 0.33 and 0.99 g/kg doses of HE were designated low (L-HE), medium (M-HE) and high (H-HE) groups,
MATERIALS AND METHODS Bacterial strains and culture conditions Two S. aureus strains were used in the present study; S. aureus 8325-4 (SigB-) and SH1000 representing a SigB+.strain. Overnight cultures were grown in Luria Broth (LB) at 37°Cwith shaking at 150 rpm. Exposure to antibiotics was carried out as detailed below. Antibiotics Ciprofloxacin were purchased from Sigma-Aldrich CO. 10 mg/ml stock solution of antibiotic were prepared freshly with 0.1N HCl and stored at -20°C. During the experiment we diluted with sterile water 1:10 and 1:100 depending on the different drug concentration.
This solution was diluted with deionized water to get sample solution of 1, 3, 5, 7ppm.0.1M solution of NaOH was used to make solution basic. Adsorbent amount was kept same for all three parameters which was 0.1g. Nano Ferrites, polyaniline, PANI nanocomposits were shaken for 30 minutes respectively. After giving contact time which was 30min for Ferrites, 30min for coated PANI/CoFe2O4 and 30min for poly aniline, sample solutions were filtered and filtrate was used to investigate the removal efficiency of adsorbent. Absorbence of all sample solution was noted using uv_visible spectrophotometer.
Then the blower was turned on for sufficient duration and UV lights were switched on for one hour. LAF microbial contamination test (Settling plate method) A total of three petri dishes were prepared aseptically inside a laminar air flow (LAF), and then the petri dish was filled by pouring sterile Tryptone Soy Agar (TSA) liquid media and allowed to solidify. One test media was placed in the LAF cabinet, one test media is placed beside the LAF cabinet while the other the test media was placed near the door. The three petri dishes lid were opened and allowed to stand for 15 minutes and closed again. Then, the test media is then incubated at 37 ° C, for 18-24 hours.
Kim et al. (2015) conducted an experiment on male mice which were randomly assigned into 4 treatment groups after 10 week period of diet-induced obesity. Four treatment groups were, a control group, a high-fat diet group, a high-fat diet group with ethanol extract from black soyabean testa (BBC), and a high-fat diet group with orlistat. BBC treatment significantly decreased feed intake. Zou et al.
Plant material and extraction 300gm dried powder of seed was weighed & taken in a aspirator (2.5L). Before placing powders into the aspirator, the jar was washed properly with acetone and then dried. 800ml of solvent i.e. methanol was added gradually. The container with its content was sealed & kept for 20 days with occasional shaking & stirring.
After solidification, 0.1ml of the inoculum was spread over the agar evenly using L rod. 6mm diameter of Whatman filter paper discs were soaked in plant extracts and dried out. Chloramphenicol and tetracycline antibiotic discs were used as the control. The discs were carefully placed on the inoculated plates and incubated for 24 hours. Later, the zone of inhibition around the disc was measured and