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Jatropha Curca Essay

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MATERIALS AND METHODS
A.IN VITRO: Effects of Jatropha curcas Seed oil as Potential Insecticide Against Pseudococcus elisae
Preparation of Jatropha Curcas Seed Oil Jatropha curcas fruits were collected in Lubo Zone 1, Santa Cruz, Davao del Sur. The fruits were brought directly to the Santa Cruz National High School (SCNHS) laboratory. The fruits were then washed with clean water and then placed into an aluminum basin. The fruits were pounded using mortar and pestle to remove the peelings. When the peelings were removed, the seeds were pounded to get the nut inside. The nuts were placed in a bottled jar. The nuts were dried under the heat of the sun for four hours. The nuts were then collected and roasted in a frying pan for five …show more content…

The set-up of the G-clamp and the metal tube were attached to a tree trunk. The nuts were placed inside the G-clamp and its tommy bar was turned that made the swivel shoe press the nuts inside in order to extract its oil. The acquired oil was placed in the reagent bottle then filtered it using funnel and filter paper. The oil was placed again in the reagent bottle.
Isolation of Pseudococcus elisae First, Pseudococcus elisae (mealybugs) were collected by the researchers in a banana (Musa sapientum) field located at Patulangon, Santa Cruz, Davao del Sur. Withered banana leaves is the indication for the presence of P. elisae. They were sealed and was brought to Santa Cruz National High School Laboratory. The P. elisae was placed in a clean petri dish by the different concentrations and covered by paper with holes. Each P. elisae was also isolated in petri dish by five then observed if it was alive by the use stereoscope. …show more content…

curcas Seed Oil against Pseudococcus elisae

Table 1 shows the average mortality rate of Pseudococcus elisae treated by different concentrations of Jatropha curcas seed oil (25%, 50% and 100%) and distilled water as the negative control in percentage after five minutes. Results showed that the 100% of J. curcas seed oil had the highest average of mortality rate with 100% compared to the 25% and 50% concentrations of J. curcas seed oil and distilled water as the negative control.

Treatment Average Mortality Rate
Negative Control(Distilled Water) 46.67
25% of J. curcas 53.33
50% of J. curcas 66.67
100% of J. curcas 100 Table 1. Average Mortality Rate of Pseudococcus elisae Treated by Different Concentrations of Jatropha Curcas Seed Oil and Distilled Water as the Negative Control in Percentage After Five Minutes

The treatment with 100% of J. curcas applied, obtained the highest average mortality rate against P. elisae among the four treatments.

A graphical representation of the average mortality rate of Pseudococcus elisae treated by different concentrations of Jatropha curcas seed oil (25%, 50% and 100%) and distilled water as the negative control in percentage after five minutes is shown in Figure

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