Diazotized Sulphanilic Acid 1. Dissolve 1.1 g of anhydrous sodium carbonate in 50 mL of water in a 100 mL conical flask. 2. Add 4 g of sulphanilic acid to the solution and heat it until it dissolves. A small amount of suspended material may render the solution cloudy.
The goal of the experiment is to synthesize a bromohexane compound from 1-hexene and HBr(aq) under reflux conditions and use the silver nitrate and sodium iodide tests to determine if the product is a primary or secondary hydrocarbon. The heterogeneous reaction mixture contains 1-hexene, 48% HBr(aq), and tetrabutylammonium bromide and was heated to under reflux conditions. Heating under reflux means that the reaction mixture is heated at its boiling point so that the reaction can proceed at a faster rate. The attached reflux condenser allows volatile substances to return to the reaction flask so that no material is lost. Since alkenes are immiscible with concentrated HBr, tetrabutylammonium bromide is used as a phase-transfer catalyst.
This experiment was done by using a mandarin orange. First, a mandarin orange was peeled off and squeezed to get 30ml of its juice in a beaker. Next, the juice was diluted with distilled water to get a measured volume of 50ml solution. After dilution, the solution was transferred into a 100ml Erlenmeyer flask. As it was done in the Experiment A, 20 drops of 0.2 M acetic acid and 10 drops of 2% starch solution was mixed well with the juice solution.
22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot plate 2. pH of the solution was adjusted to 7.0 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Lauryl Sulphate Broth
Standardization of NaOH solution Accurately weigh out a sample of approximately 0.3-0.4 g of primary standard potassium hydrogen phthalate, KHPh, which has been previously dried at 120°C. Do not use more than 0.4 g. To obtain an accurate mass, weigh the sample on weighing paper, slide it into a clean (but not necessarily dry) 250 mL Erlenmeyer flask and reweigh the paper to account for any KHPh that may remain on it. Dissolve the KHPh sample in about 50 mL of CO2-free water and add 2-3 drops of 0.1% phenolphthalein indicator. Begin adding the approximately 0.1 M sodium hydroxide solution from the buret while continuously swirling the flask contents. Do not open the stopcock completely.
One of the reactions you observed resulted in this product: NaCl + H2O + CO2 (g)? What well did this reaction occur in? Describe how the observations for this reaction support your answer. B BoldI ItalicsU Underline Bulleted list Numbered list Superscript Subscript70 Words A reaction I observed in number 1.) Sodium Bicarbonate mixed with Hydrochloric acid.
The sample was transferred to a 250 ml conical flask kept in water bath for alkali treatment. 75 ml of 17.5% caustic soda was measured using a measuring cylinder at 20°C. 15 ml of 17.5% NaOH was added and fibres were macerated gently with a flattened glass rod for 1 minute. 10 ml more NaOH was added and the solution was mixed for 45 seconds. 10 ml NaOH was again added and mixed for 15 seconds to make lump free slurry.
Here we will be using baking soda and vinegar which are our two components which will cause a chemical reaction. Reactions can be affected by several things such as temperature, concentration and the presence or absence of a catalyst which is a substance which can increase the rate of the reaction. To determine the rate of a reaction there are many methods that can be used. For example. A method which will be used in this experiment will be to use a gas syringe to measure the volume of the gas which has been produced.
Using a permanent marker draw a line on the 0% sugar solution bottle at the water level. Measure out the same line on the other 5 bottles labelled 5% sugar to 25% sugar. This line will indicate the maximum volume of water when filling the bottles after the sugar has been added to the empty bottles. Measure out and add the following amounts of sugar to each bottle as outlined in the steps below: - 0% sugar – 0 grams of sugar - 5% sugar – 100 grams of sugar - 10% sugar - 200 grams of sugar - 15% sugar – 300 grams of sugar - 20% sugar – 400 grams of sugar - 25% sugar – 500 grams of sugar Fill the other 5 bottles labelled 5% sugar to 25% sugar with water up to the marked line. Put the caps on the bottles and shake them until all the sugar is dissolved.
Next, transfer the sample to a 50 mL beaker and mixes with distilled water, which gets by rinsing the crucible with its cover in 8mL, so the solution is generated. After that, put an aluminum wire into the beaker, and after a certain period of time the solution gains color. To finish the reaction, 5 drops of 6M of Hydrochloric acid is added into the beaker to clean the solution, which means that acid dissolves all salts of aluminum that is on the solution. After finishing the chemical process, collect and use the Butcher funnel to wash the cooper because it is going to be used to a vacuum filtration. After finishing the filtration, measure the weight of the sample and dry it.
15. Add another 25cm3 of Methanol and Ethyl acetate to the solutions. Stir gently for 20 minutes using a stirring rod this is to allow more of the active ingredients to mix with the solution. 16. Take two funnels and place one in two separate clean measuring beakers making sure the bottoms of the funnels don’t touch the bottom of the measuring beakers.
While swirling the phosphoric acid solution in the Erlenmeyer flask, the sodium hydroxide solution was added to it a few drops at a time using a disposable plastic pipette. The After all the sodium hydroxide was transferred, the flask was rinsed with 2 mL of deionized water and added to the flask with the reaction mixture and swirled for an additional minute. A clean, dry evaporating dish with a watch glass was then weighed and recorded to 0.001 g. The reaction mixture was then transferred to the evaporating dish. The flask was then rinsed with 2 mL of deionized water and added to the evaporating dish containing the reaction mixture.
Two chemical reactions are carried by adding sodium hydroxide to the acidic solution from Part I. During the first reaction is the neutralization of the excess of nitric acid in the mixture by sodium hydroxide. The second reaction takes the place after naturalization is a complete and NaOH is in excess. While the liquid inside the beaker is being stirred, with the stirring rod, 10 ml of 6 M NaOH is poured into the solution from Part I at 1 mL at a time. After each 1 mL the solution is tested for acidity with red litmus paper.
32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate. 50 μL of these dilution solutions were separated on the TLC plate coated with SNISG. The plate was developed with petroleum ether: ethyl acetate (4:1) and the movement of solvent was usually controlled at 1 cm from the upper edge. After completion, the plate was dried until no solvent smell remained. It was sprayed with an ethanol solution containing 10% sulfuric acid, and heated at an infra-red drier until obvious color came up, as shown in Fig.2 (B.ab).