Kombucha Case Study

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Chapter V To study the mechanism of action of Kombucha 5.1: To study the mechanism of action of Kombucha by assessing peripheral utilization of glucose using rat hemidiaphragm Introduction Energy homeostasis is maintained by the integrated role of multiple organs and tissues such as the digestive system, pancreas, liver, muscles, adipose tissue and brain. Defects in the sensing energy needs, and the ability to respond appropriately, result in metabolic diseases such as type 2 diabetes mellitus. Insulin is responsible for the conversion of simple monomers such as monosaccharides and amino acids into complex macromolecules such as proteins, lipids and glycogen. This is mainly achieved by increasing glucose uptake in liver, muscle and adipose tissue. The liver plays a unique role in normal physiology of the entire systems of the body. In postprandial nutrient metabolism it has first access to most ingested and absorbed nutrients. One of the most important roles of liver is to store and release glucose in fed and fasted conditions. In normal conditions, excess glucose after intake of food substantially leads to storage of glucose as glycogen in the liver. The majority of insulin-stimulated glucose uptake occurs in skeletal muscle (Leto and Saltiel, 2012), where glucose is stored as glycogen, which is mobilized when there is a demand of energy and low glucose content. About 10% of insulin-stimulated glucose uptake occurs in adipose tissue, where it is stored as

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