Catalase Experiment Can hydrogen peroxide be broken down by catalyst other than those found in a living system? Independent and Dependent Variables Independent Variable: The independent variable is the chicken liver and the potato. Dependent Variable: The dependent variable is what I will be measuring which is how much peroxide I will use for each individual experiment. I will also need to measure the temperature of the peroxide during/after the experiment and the pH balance. Purpose “What was the purpose for this lab?”- IRP Instructions The purpose for the Catalase Lab Experiment to have figured out if Hydrogen Peroxide could be broken down by catalyst found in and not in a living system. Background Information Biological catalyst have enzymes which contains …show more content…
A piece of Chicken Liver was warmed. Other piece was left cold. A piece of Potato was warmed. Other piece was left cold. A 2.54x2.54 cm of warm Chicken Liver was placed in a 16x100mm Test Tubes numbered 1. A 2.54x2.54 cm of cold Chicken Liver was placed in a 16x100mm Test Tubes numbered 2. A 2.54x2.54 cm of warm Chicken Liver was placed in a 16x100mm Test Tubes numbered 3. A 2.54x2.54 cm of cold Chicken Liver was placed in a 16x100mm Test Tubes numbered 4. A 2.54x2.54 cm of warm Potato was placed in 16x100mm Test Tubes numbered 5. A 2.54x2.54 cm of cold Potato was placed in 16x100mm Test Tubes numbered 6. A 2.54x2.54 cm of warm Potato was placed in 16x100mm Test Tubes numbered 7. A 2.54x2.54 cm of cold Potato was placed in 16x100mm Test Tubes numbered 8. 113.398 g of 3% Hydrogen Peroxide was placed in a 16x100mm Test Tube labeled 1. 113.398 g of 3% Hydrogen Peroxide was placed in a 16x100mm Test Tube labeled 2. 113.398 g of 3% Hydrogen Peroxide was placed in a 16x100mm Test Tube labeled 5. 113.398 g of 3% Hydrogen Peroxide was placed in a 16x100mm Test Tube labeled 6. 113.398 g of Apple Cider Vinegar was placed in a 16x100mm Test Tube labeled
Example of enzymes involved in biological processes are classified into; oxidoreductases, transferases, hydrolases, lyases, isomerase and ligases. Catalase an oxidoreductase and among the vital enzymes in the body, it catalyses the breakdown of hydrogen peroxide
Procedures In this experiment, an unknown mixture was given, that contained both sodium bicarbonate(NaHCO3) and sodium chloride(NaCl). The purpose of this experiment was to find the percent (%) composition of the sodium bicarbonate and sodium chloride in the unknown mixture. Hydrochloric acid(HCl) and a scale were materials that were also used during the lab.
Introduction The purpose of this lab was to use chemical and physical tests to identify indicators of disease in synthetic urine samples. This lab tested samples for protein levels, glucose levels, and pH levels. In a normally functioning individual, proteins cannot pass through the glomerulus; therefore proteins should not be found in urine. However, in the nephrons of individuals with Bright’s Disease, the glomerulus no longer stops all proteins from entering the urine (Giuseppe et al., 2002, pp.
Using two separate aseptic pipettes, 250 µl of LB broth were added to each micro test tube and mixed gently. Likewise, using two separate, aseptic pipettes for each tube, 100 µl of solution was added to the appropriate agar plate. After, using a new loop for each plate, the solution was spread gently across their surfaces. Lastly, the plates were stacked, taped together, and labelled before placing them upside down in an incubator set at 37°C
Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.
For instance, we could not conclude that mitochondrial activity is present in Supernatant II. However, our experiment showed that the boiled corn kernels did not undergo any mitochondrial activity while the raw corn kernels did. This might indicate that raising the temperature might have an effect on the function of dehydrogenase. Moreover, our found that starch granules are present in both sediment I and the “gunk”. Indeed, some parts of this experiment were not successful because the procedure was not followed
1. Technology map 1.1. Description of the technology In-vitro meat (also known as cultured meat or animal-free meat) is an innovation based on the idea of manufacturing meat products by applying tissue-engineering technology. The process consists of extracting starter cells of animal origin and growing them artificially in a synthetic culture media through self-organization or scaffolding techniques (Futurefood.org, 2015).
Enzymes are a form of protein that lowers activation energy and speeds up reactions as a catalyst. They are made by the stringing together of an abundant amount of amino acids and folded into a specific shape for chemical reactions. Turnip Peroxidase is the enzyme used in this lab and is derived from the vegetable. Enzymes are not used up or permanently altered by their environment Peroxidases are found in a range of organisms and function to break down alcohol (H2O2) and creates byproducts of oxygen and water. In this experiment, the reducing agent guaiacol is added with the substrate, hydrogen peroxide, to create water and oxygen.
Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
Materials: The materials that I will be utilizing during these experimentations are three to four ice cubes, one cup for measuring, six unblemished cups, one stopwatch, one hot water source, three tablets of Alka-Seltzer, one thermometer that measures from negative
Why is it a good idea to pull data from the entire class? It is a good idea because it allows you to see other results and to prove your results are correct. 2. Look at the graph for part A , how does the enzyme activity vary with concentration? The more concentrated the greater the reaction.
Independent Variable amount of substrate (sucrose) present 3. Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. I believe glucose and fructose was used as an indicator because they are what produces sucrose and sucrose creates more sucrase activity.
Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of
Bio Chem lab Report 04 Enzyme Biochemistry Group Member: Chan Man Jeun Duncan (16002621) Law Sze Man (16000478) Introduction Enzyme is a protein base structure substance in our body. It works at a biocatalyst that will catalyzing the chemical reaction, which helps to speed up the chemical reaction. Enzyme could only function in specific shape, and the shape of enzyme is depending on the environment, therefore it is hard for an enzyme to function well in an extreme environment. The aim of this experiment is to see can the enzyme functions normally in different environment(pH, temperature and salt concentration) via using starch solution, amylase from saliva, 0.5M HCl solution, 0.5M NaOH solution and NaCl solution, and using iodine solution
They can only quicken reactions that will eventually occur, but this enables the cell to have a productive metabolism, routing chemicals through metabolic pathways. Enzymes are very specific for the reactions they catalyze; they make sure the chemical processes go in the cell at any given time. Peroxidase was the enzyme being testing in this experiment. A peroxidase is an enzyme that acts as catalysts, which occurs in biological systems. Peroxidase is found in plants, which they play a role in helping to minimize damage caused by stress factors or insect pests.