As a part of our study on how to completely extract trimyristin from nutmeg, we had to determine the best way to find a solvent that would properly recrystalize it. We used triphenylmethane as a test compound, attempting to find a solvent that would allow it to recrystalize. The most suitable solvent would dissolve the solute when the solvent is hot, not dissolve it when the solvent is cold, and should be fairly volatile and easily removable from the desired product. We tested water as a potential solvent and triphenylmethane would not dissolve when the water was at room temperature or when it was hot, so this was not a good solvent. When we tested methanol, triphenylmethan did not dissolve at room temperature, but did dissove when the methanol was hot, and then recrystalized into a solid when the mehtanol was cooled, so this was a suitable solvent.
2.4.1. Tetramethyl glucose acetylation 1gm of tetramethyl glucose was dissolved in 5ml of acetic anhydride and added to fused sodium acetate of 0.375gm and mixed for 10 minutes and allowed to cool. To this mixture 7.5ml of toluene and 5ml of dry ether were added. The whole mixture evaporated to syrup on a water bath at 50 °C. The product dissolved in the dry ether after washing with toluene.
5 mL of 3M sodium hydroxide, 5 mL of de-ionized water, and 15 mL of hexane were added to the reaction flask and stirred. The mixture was transferred to a separatory funnel, separated into an organic layer and water layer, and then drained. The water layer was washed twice with 10 mL of hexane. The organic layer was dried
For example, an experiment similar to this one could be how pressure could affect the time it takes for an Alka-Seltzer tablet to dissolve. Using A flask and rubber stoppers, you can compare the amount of time it takes for and Alka-Seltzer tablet to dissolve in regular room temperature water and room temperature water in a flak but with a rubber stopper stopping the air flow into the flask. This is related to the experiment performed above because the increase in pressure from the rubber stopper stopping the air flow in and out of the flask may affect the time it would take for the Alka-Seltzer tablet to dissolve. Another example of an experiment related to the one performed above is how the amount of water used to dissolve an Alka-Seltzer tablet effects the time it takes for one to dissolve. This also relates to the experiment performed above because it also affects how fast the rate of reaction is.
N-(1-Carboxymethyl-1H-tetrazol-5-yl)-hydrazinium nitrate (3). A solution of AgNO3 (0.10 g, 0.60 mmol) in distilled water (1.5 mL) was added dropwise in the dark to the solution of compound 2 (0.10 g, 0.60 mmol) in Deionized water (1.5 mL) under stirring. After 2-3 hour, the precipitate was filtered, and rinsed with 4 mL distilled water. The solvent was removed by rotary Evaporation to produce a white solid at 88% yield (0.10 g); N-(1-Carboxymethyl-1H-tetrazol-5-yl)-hydrazinium nitrate: Yield: 88%; yellow crystals;. IR (KBr): 3396, 3329, 3140, 3008, 1628, 1494, 1383 cm-1; UV (H2O): λmax = 293-296
Figure 2. Column chromatography set-up After setting up the column, 2 10-ml of the chosen solvent was obtained and was placed in two separate test tubes. Using a dropper, ~0.5 mL of the food dye was put into the column by dropping it at the side of the column in a circular motion. The chosen solvent was then added just after the green food
The natural agar consist of Yeast Extract, Tryptone , Lactose , Manniiol, Sodium Chloride , Dip otassium Hydrogen Phosphate ,Gelatin and Agar .Then , in a conical flask 14g of nutrient agar was mixed into 500 ml of distilled water and , The mixture was stirred and dissolved until most of the agar dissolve. By non-absorbent cotton wool plug the mouth of the flask was closed. By using the autoclave the agar was sterilized for 15 minutes.The cotton wool was removed. By the flame the mouth of the flask was heated before and after pouring the agar into the Petri dishes. And, the left hand the lid of the Petri dish was lift, just enough to enter the mouth of the flask and quickly was poured in agar (about 15 cm3).
On the second day of incubation, the plate was removed from the incubator and placed over a hot plate heating Iodine solids. The smoke of the Iodine stained the plate to display the presence or absence of a halo around the bacteria 2.12 Lipid Hydrolysis This test was done by making a single line streak inoculation on a tributyrin agar plate and allowing incubation. After the incubation period, the plate was observed for the presence or absence of a halo around the bacteria. 2.13 Gelatin Liquefaction A gelatin deep was deep stabbed and incubated. After incubation the tubes were placed in 4ºC for 30 minutes.
Wash ether layer with saturated sodium chloride solution and retain ether layer. In a small 125ml Erlenmeyer flask, dry the ether solution over anhydrous calcium chloride. Add sufficient calcium chloride so that it no longer clumps to pellets added earlier on the bottom of the flask. Remove the solvent using a rotary evaporator and weigh product. Results 1 mole of benzoic acid (C6H5COOH = 122.12grams) reacts with 1 mole of methanol (CH3OH = 32grams/mole) to produce 1 mole of methyl benzoate (C6H5COOCH3 = 136.15grams) and 1 mole of water.
SDS-Polyacrylamide gels were prepared and the glass plates were washed with 70% ethanol and water. After drying the plates, water was used for test leakages. Two SDS-Polyacrylamide gels were prepared according to the following recipe. These all above components of the running gel were added in a 50 ml tube and solutions were mixed and pipetted into the prepared gel chambers. Glass plates were filled ¾ and the gel was covered with 100-500 µl Isopropanol in order to achieve an even surface.
Two chemical reactions are carried by adding sodium hydroxide to the acidic solution from Part I. During the first reaction is the neutralization of the excess of nitric acid in the mixture by sodium hydroxide. The second reaction takes the place after naturalization is a complete and NaOH is in excess. While the liquid inside the beaker is being stirred, with the stirring rod, 10 ml of 6 M NaOH is poured into the solution from Part I at 1 mL at a time. After each 1 mL the solution is tested for acidity with red litmus paper.