Lab Report: Isolation Of Streptomyces Sp

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Isolation of Streptomyces sp
The soil samples were collected from various agricultural areas which had been serially diluted up to 10-5. The spread plate technique was followed and incubated at 28°C for 7 days. The Streptomyces sp isolates were maintained on starch casein agar slants.
Screening for antimicrobial activity by well diffusion method
The Streptomyces isolates were grown in starch casein broth at 28ºC for 10 days and the activity were observed by well diffusion method. The culture filtrate was loaded into the wells which had been inoculated with test organism, S.aureus, K.pneumonia, P.aeruginosa and E.coli and incubated for overnight and measured the zone of inhibition.
Extraction of the secondary metabolites from culture supernatant using different solvents
The bioactive compound from crude culture broth was extracted with different solvents like n- butanol, chloroform, ethyl acetate, n- hexane and petroleum ether. The solvents were mixed in the ratio of 1:1 and tested for the presence of secondary metabolites using well diffusion method. The best activity showed extract was chosen for minimum inhibitory concentration (MIC).
Minimum inhibitory concentration (MIC)
The lowest concentration of bioactive compound that inhibited the growth of microorganisms was considered as the minimum inhibitory concentration (MIC value). The MIC value of the Streptomyces extract was determined by broth dilution method. The Streptomyces extract was gradually diluted in Muller

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