Enterococcus Faecium Lab Report

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Lab-experiment immunity and bacteria- How do they react?

Research question: How does the bacteria Enterococcus Faecium SF 68 demonstrate resistance against the following antibiotics: Oxacillin, Climdacylin, Penicillin-G, Amikacin, Lincocymin, Erythromycin, Cephazolin, Mezlocillin ?

Terminology used:

Bacterium: Singular form of bacteria, one single individual. A bacterium an organism that possesses one single cell and is very adaptable to most environments. A bacterium contains only a single chromosome, but posses more sections of DNA known as plasmids, that are spreading all around the bacteria in an area called the cytoplasm.
A bacterium is classified as a Prokaryote. A bacterium grows best when the temperature of its environment
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Your Agar plate should now be sterilized, the process of sterilization should be finished.

Procedure of the experiment:
Prepare all the test tubes and place them in their respective test-tube holders.
Put 10 millimeter of the 0.9 % saline solution in one test tube.
Put 10 millimeter of the chicken broth in the other test tube.
Put 1 capsule of the bioflorin into each of the test tubes and tap them until the capsule dissolves.
Screw the liquid onto both test tubes to make sure that they are sealed.
You now have to wait for approximately two days, in order to obtain satisfying results.
Light the candle/put it on fire.
Fill the third test tube with approximately two millimeters of Ethanol.
Dip the inoculation loop into the test tube containing the alcohol
Hold the inoculation loop over the flames of the candle for just a second of time until all alcohol is combusted.
Unscrew the lids of the other two test tubes, while still holding on to the inoculation loop.
Dip the inoculation loop into the chicken broth and then into the saline solution.
Close the test tube containing the chicken

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