22.5 g of plate count agar powder was dissolved in a litre of sterile distilled water on the hot plate 2. pH of the solution was adjusted to 7.0 ± 0.2 by adding NaOH or HCl and was immediately transferred into the Schott bottle to be autoclaved at 121 ° C for 15 minutes 3. Prepared medium was stored in 4° C chiller Lauryl Sulphate Broth
The formation of a dye-iodine complex will occur in the cytoplasm. Then, it was flooded with ethanol and washed immediately. It is where the process of decolorization occurs. It should not be prolonged as it can over decolorized and immediate washing would sometimes cause under decolorized smear and. Finally safranins were flooded on slide for 30 seconds and rinse it with tap water and the slide must be dried using a blotting paper before viewing and examine in the
A sufficient amount of this solution was poured into two petri dishes labelled “LB -pGLO” and “LB”. Into two dishes labelled “LB/amp -pGLO” and “LB/amp +pGLO”, the agar broth with ampicillin was poured in. Arabinose C sugar was then added to the broth and was poured into one dish labelled “LB/amp/ara +pGLO”(Fig. 1). They were left overnight to harden.
Carefully remove the comb and tape surrounding the plate and transfer the gel plate to the electrophoretic tank such that wells are towards the cathode 4. Pour 1X TAE buffer into the tank until the gel is completely submerged. Connect the electrode to the power supply 5. Load the plasmid DNA preparation and standard DNA marker into separate well with help of a micropipette or a syringe 6. Turn ‘ON’ the power supply and run at 100 V (10-15 mA).
The media used in this experiment was Trypticase nitrate broth. The reagents used (A and B) were sulfanilic acid and alpha-naphthylamine (respectively). Using aseptic technique, the bacterium (16A and 16B) were inoculated into labeled broth test tubes. The tubes were incubated for 48 hours at 37 degrees Celsius. When the incubation was complete 5 drops of reagent A and 5 drops of reagent B were added to each of the broths.
Preparation of hot water extracts: 20 g from each bark powder was surface sterilized and mixed with 100 ml sterilized water and placed in water path for 90 minutes at 100ºC, and then filtered through 3 layers of sterilized cheese cloth. The products were kept in a refrigerator at 4 ºC until needed for testing their bacterial
Once each well was filled, a stopwatch was prepared to time the reactions that would occur in the next steps. To begin the reactions, the “Na2S2O3” syringe was used to place 2 mL of Na2S2O3 into Well #1. As soon as the sodium thiosulfate was placed in the well, the stopwatch was started. The reaction was timed until the black “x” beneath the well was no longer visible. Once this point was reached, the timer was stopped and the time was recorded in Data Table #1.
The chemical compound and the water were mixed together using a glass rod until the chemical was thoroughly dissolved. After the chemical had dissolved, the beaker containing 20mL of room temperature water was placed into the chemical mixture with a thermometer in the beaker as well. The lid for the polystyrene cup was then placed onto the cup, and the stopwatch began timing. The lid was not removed until exactly 2 minutes had passed, and the thermometer was taken out. The final temperature was recorded and the difference in temperature change was calculated by subtracting the initial water temperature from the final water temperature.
Fe3O4 nanoparticles were synthesized according to our previously reported method by chemical co-precipitation of Fe2+ and Fe3+ ions with a molar ratio of 1:2 . Briefly, 2.4 g of FeCl3.6H2O and 0.8 g of FeCl2.4H2O were dissolved in 30 mL of deionized water under using continuous N2 purge at 70 °C. and Under vigorous stirring, followed by dropwise addition of 10 mL of NH3.H2O was dropwise added to the reaction mixture until the color of mixture turned to black and kept reacting for 90 min to complete the reaction. At the end, the synthesized Fe3O4 nanoparticles were separated by a magnet and washed by using water and ethanol for further three times with 89.3%