Biomolecules: The Four Types Of Carbohydrates
Introduction:
Biomolecule is a macromolecule that exists in either as protein or nucleic acid in living organism. Carbohydrates are one of the examples of macromolecules that made of carbon, hydrogen and oxygen only. It derive its’ name from general formula (CH₂O)n where n is more or equal to 3. Carbohydrates can store as starch in plants and glycogen in animals can also be found in milk, bread, rice and etc. It can be classified into four types which are monosaccharides, disaccharides, oligosaccharides and polysaccharides. Monosaccharide is the simplest sugar and the smallest that made up molecules of carbohydrates for example are glucose, fructose and galactose. Condensation of two molecules of monosaccharides will form disaccharides which are
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Barfoed’s test
Objective :
To differentiate between monosaccharide and disaccharide.
Sample of carbohydrates: Sample A, B, C and NEGATIVE CONTROL
Methodology :
1. 1ml sample and 1ml of Barfoed’s reagent are added in each test tubes.
2. The test tubes are then heated in boiling water for three minutes.
3. Each test tubes are cooled down for two minutes through running tap water.
Result :
Sample The presence of brownish-red precipitate
A Presence
B Absence
C Absence
Negative control Absence
Discussion :
The reaction between Molish test and the sample will give a positive result if the purple ring is formed between the layers of the sample. In this experiment, sample A, B, C and D give a positive result, while negative control shows a negative result. Purple ring between the layers of the sample shows the presence of carbohydrates.
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