Qualitative Synthesis Lab Report

3- Add 0.05 moles of concentrated sulphuric acid to round bottom flask very slowly and carefully then put a magnetic stirrer . 4- Set up reflux system using a clean and dry condenser .

We used a Buchner funnel to collect benzocaine. We used three 10 ml of water to wash the product. After the product was dry, we weighed, calculate the percent yield and determined the melting point of the product.

Equation 1: Absolute Error = Experimental Value – Accepted Value Equation 2: Standard Deviation: Equation 3: Density (ρ) = Mass (m) / Volume (V) Equation 4: General Methods and Materials:

13. Read the volume from the bottom of the meniscus. 14. Swirl the solution to ensure that the oxalic acid crystals are properly dissolved in the deionised water. C)

 Dissolve the salt in 60 ml of tap water. Add 30 ml 6 M Hcl and stir the mixture with a glass rod.  Add 12 g solid Nacl to the solution and stir the mixture for about 2 minutes.  Support a 250 ml separatory funnel on a ring, making sure that the stopcock is closed and that a clean beaker is placed beneath the exit tube. Transfer the aqueous solution from the beaker to the separatory funnel.

In 10 g dried sediment sample added 7 ml 0.2 M NH4Cl solution. A mixture of 100 ml hexane: acetone (1:1) was used as a solvent to extract pesticides with overnight shaking for 12 h on reciprocal or wrist action shaker at 180 rpm. The extract was carefully decanted through activated florisil column (2-3 cm), giving twice wash with25 ml hexane: acetone (1:1) to the sediments. The elute was then washed with 200 ml water and then again aqueous layer was extracted with 50 ml hexane. Finally the hexane layer was washed with 100 ml water and then evaporated to dryness with a vacuum rotary evaporator.

Empty the blank and use the solution from test tube one to rinse the cuvette twice. Fill it ¾ with solution one, wipe the outside, and place it in the spectrometer. 8. Start data collection and display a full spectrum graph. Stop the data and the wavelength of maximum absorbance will be identified.

Apparatus- Chromatography column: C18 (10 microns particle size), with Guard column Flow rate: 1.2ml/min Pressure: 30-40kgf Wavelength: 326nm Mobile phase: methanol : water (95:5 v/v) Internal standard: retinyl acetate Injection volume: 20µl Procedure for Retinol extraction from serum samples- 1) 100 µl of serum sample and 100 µl of Retinyl acetate were added into 12 X 100mm glass test tubes. Vortex-mixed for 30 seconds. Then, kept them at 4 C for 5 mins. 2) 1mL of hexane was added and vortex-mixed intermittently for 60 sec.

Then, the test media is then incubated at 37 ° C, for 18-24 hours. Rinsing reusable instruments The samples were rinsed with 40 ml of pyrogen-free water using a glass beaker that is free from pyrogens. Endotoxin testing using STV

Making tea solution: Take around 3 gram of tea from tea bags and record the weight with an uncertainty of ± 0.001 using an high accuracy balance. Take 400 ml beaker add 200 ml of distilled water to it. Start heating it up with the bunsen burner until 150ml remain. (recommended ratio of 1 g of tea : 50ml of water) Measure the temperature of water and wait till it reach the expected value Add the tea leaf once the water is 150ml(some will evaporate during the heating) and keep the temperature at a constant degrees by using a water bath. Using a stopwatch to determine the amount of heating time.

In a large sauce pan, combine milk, sugar and salt and bring to a boil over medium heat. Boil for 8 minutes, while stirring. 3. Remove the pan from heat and stir in marshmallows, chocolate, lime zest and lime juice. Stir until the marshmallows and chocolate are melted and the mixture is smooth.

Before starting the heating process, measure the weight of the crucible with its cover first and then tare the balance, and after that adding about 1 gram of the sample to the crucible with its cover, and then weigh it. Moreover, it is possible liberating harmful gases during the process of heating; therefore, being careful is important. The heating process ends when this sample changes the color to brown because water of hydration is removed to the sample. Additionally, give time to the small cool down and measure its weight. Next, transfer the sample to a 50 mL beaker and mixes with distilled water, which gets by rinsing the crucible with its cover in 8mL, so the solution is generated.

The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.