Optimization of cultural parameters for the production of antimicrobial compound from Lactobacillus fermentum (MTCC No. 1745) Sri Santhi Lanka1 and VSSL Prasad Talluri2 1. Department of Biotechnology, GITAM Institute of Science, GITAM University Visakhapatnam-530045,India. 2. Assistant Professor, Department of Biotechnology, College of Natural and Computational Science, University of Gondar, Gondar, Ethiopia *Corresponding author: Sri Santhi Lanka, E.mail srishanthichowdary@gmail.com Abstract: To improve the productivity of antibacterial compounds of Lactobacillus fermentum by optimizing its nutrient and physical factors and screened for its antimicrobial activity by agar well diffusion method. In order to improve its efficiency, …show more content…
Determination of optimum concentration of best nitrogen source The maximum production of Zone of inhibition shown by nitrogen source was further optimized by altering its concentration from 0.5% to 3.0%, to determine the optimum concentration. Effect of pH To evaluate the effect of pH on growth and zone of inhibition was determined by changing the pH (5.0 to 9.0) by adjusting to required value by addition of 1 N HCl or 1 N NaOH of the optimized media containing best carbon and nitrogen source. Effect of temperature The optimized media containing best nitrogen, carbon sources at optimum pH was incubated at various temperatures ranging from 20 ºC to 50 ºC, to determine the optimum temperature required for maximum growth and production of Secondary metabolite. Effect of incubation period The optimum incubation period required for the growth and production of Zone of inhibition was determined by incubating the optimized media with best carbon, nitrogen and amino acid sources at optimum pH and temperature at different incubation periods (12 h to 96 h). Statistical
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
K.D.A. Saboia et al. , (2007) have been prepared the Bi4Ti3O12–CaCu3Ti4O12 {[BIT(X)–CCTO(100-X)]} composite powders through solid state reaction method and calcined in the range of 900 to 1020 ºC for 12 h. The as-prepared powders have modified in the form of thick film onto alumina ceramic substrate by utilizing screen printing. At 100 Hz, the value of dielectric constant (κ) of CCTO100 and BIT100 is 316.61 and 53.64 respectively. Conversely, the composite with X=20 % shows an unexpected dielectric constant of 409.71, which is around 20% higher in comparison with the CCTO.
The purpose of this experiment is to create a complete genomic library of Aliivibrio fisheri through the use of the lux operon. The examination of the lux operon gene occurs through the extraction of the DNA of Aliivibrio fischeri and digest a large piece of DNA to smaller random pieces. The fragment of DNA will later be ligated together in plasmid. Plasmid acts as vectors to transport DNA from one organism to another. The DNA will then run through a UV-visible spectrophotometer to test the absorbance of the extracted DNA.
Name: Avishak Deb Roy Partners: Leevell Penn, Varugh, Butler Bio 101 Lab Report #1 02.22.2018 Swimming speed of paramecium tetraurelia in different levels of treatment. Introduction Paramecia is a unicellular Protista which are naturally found in aquatic habitats. It is easily cultured in the laboratory. It is oblong shaped and covered with short hairy structure called cilia. Paramecia does not pose any health or ethical concerns and the population can be maintained if there is a food source such as Enterobacter (Biological Foundation 7).
In this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections.
Under anaerobic conditions, alcoholic fermentation occurs in Fleischmann’s Rapid-Rise yeast (Saccharomyces cerevisiae). During glycolysis, glucose is converted to two pyruvate; consequently, the end products are two ATP molecules from substrate level phosphorylation and two NADH molecules. Then, NAD+ regeneration occurs, first converting two pyruvate to two acetaldehyde while also releasing two carbon dioxide molecules, and ultimately two acetaldehyde converting to the end product of two ethanol with the regeneration of two NAD+. It is important to indicate that the alcoholic fermentation process can be applied to the use of ethanol as biofuel. With this in mind, ethanol biofuel is expected to produce lower levels of pollution with the use
Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Tests: Lactose fermentation: Fermentation makes energy available for use by microorganisms by anaerobic breakdown of carbohydrates. The product can either be an acid or gas. When it is positive, the broth will turn from red to yellow and if gas is present a bubble is formed.
1. Experiment, relationships, & Explanations: 1.1. Phenomenon under study • Newton’s Third Law of Motion • Pressure 1.2. Hypothesis or hypotheses • Allia: My hypothesis is that after I let go of the carton and remove my hand that is covering the bottom holes that one, the water will begin to flow out of the holes.
Introduction The purpose of this practical is to purify and detect lysozyme from a mixture of components. Lysozyme is a protein found in high concentration in egg whites and is found to be very useful in the pharmaceutical and food industry as it has a high anti-microbial, anti-viral and anti-parasite properties. In hens, it is said to account for 3.5% of the total protein in a hen egg white.
Cellular Respiration Lab Introduction In this lab, the primary investigation was to discover which factors affect cellular respiration. In this particular inquiry, the factor tested was the amount of time the lentil seeds were germinated. This study was performed in order to understand the process of cellular respiration as well as be able to measure and observe gas concentration as a result of impacting factors. Cellular respiration is necessary for life-processes, converting glucose and oxygen into ATP, carbon dioxide, and water, in a series of metabolic reactions.
Results Part 1: Effects of Heat on Bacterial Growth Table 1. Bacterial Growth Based on Heat 40 °C (Group 1) 55°C (Group 2) 80°C (Group 3) 100°C (Group 4) and (Group 5) Time (min) 10 20 30 40 10 20 30 20 30 40 10 20 30 40 Escherichia coli X X X
Title: How Ph Levels Affected the Fermentation of Beer Hypothesis: The beer will be left with more sugar deposit as the Ph levels increase because alpha/beta -amylase will no longer function. Predictions: Alcohol Percentage Analysis for the Control and the Experimental During this experiment, the pH level was increased, therefore Alpha-Amylase was favored. Due to the nature of Alpha-Amylase cutting randomly through a large carbohydrate molecule, it leaves bigger sugars in the flask, which cannot be digested by yeast. Due to this, less reactions should occur in the experimental, therefore leading to a lower percentage of alcohol production, compared to the control.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
The media used in this experiment was Trypticase nitrate broth. The reagents used (A and B) were sulfanilic acid and alpha-naphthylamine (respectively). Using aseptic technique, the bacterium (16A and 16B) were inoculated into labeled broth test tubes. The tubes were incubated for 48 hours at 37 degrees Celsius. When the incubation was complete 5 drops of reagent A and 5 drops of reagent B were added to each of the broths.