Lb Agar Essay

LB agar was divided into 4 quadrants. First quadrant, which was labeled with C, shows the microbial colonies found on coke. After culturing ,only 1 colony was found on coke. İt has pale yellow colour, circular shape and medium size. 1 colony was seen on money too. It has a same properties with colony found on coke. 2 different types of bacterial colonies were seen on thumb of the washed hand. arrow 4 and 5 indicates the bacterial colonies which were found on washed hand. Arrow 4 from figure 1 shows 3 colonies with yellow color, medium size and circular shape. Arrow 5 represents 8 colonies. They have white color, including both small and medium sizes, and punctiform On the other hand, more bacterial colonies were seen on unwashed hand as

ıt is easy to prepare. It provides the environmental and nutritional conditions similar to their natural habitat. It is useful when a specimen contains a mixed bacterial colonies to achieve distinct colony-forming units. It also has disadvantages like it does not support the simultaneous growth of aerobic and anaerobic bacteria. Consequently, duplicate plates must be inoculated, and incubated in both atmospheres. Solid media cannot accommodate large inocula volumes, typically those greater than 0.05ml. Because plate media are shallow and have large surface areas, they can dehydrate quickly and lose their ability to support growth within a few days if evaporation prevention measures are not taken.

Blood agar is an enriched, bacterial growth medium. Blood agar is a type of growth medium (trypticase soya agar enriched with 5% Sheep blood) that encourages the growth of bacteria, such as streptococci, that otherwise wouldn’t grow well at all on other types of media. Blood agar has two major uses: To grow streptococci and to determine the type of hemolysis, if any. It can be used to distinguish between groups of organisms or species; it is therefore a differential medium. It is important to examine the area around an isolated colony for each