3. INTRIDUCTION
In this investigation project I was testing to see which of my potatoes will prefers to grow in favorable conditions (dark, moist places) or if they will grow in non-favorable conditions (lemon juice). I wanted to see if the potatoes will grow mould on them while being in the lemon juice and in a dark moist place for a week (7 days). However, my potatoes rather grow in a non-acidic to low acidic, dark, moist, high in protein place because it thrives in those conditions better. Mould is bacteria that has grown on the dead, it is saprophytic which means they live off the dead as a source of food supply. When I was doing my research about the conditions that bacteria thrive in is 4.4˚C - 60˚C.
4. LITERATURE RESEARCH
Source
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PROBLEMS BEFORE INVESTIGATION
• Do the containers that the potatoes will be in have to be clean to achieve accurate results?
• How often would I have to observe the potatoes?
• Will the results of the potatoes differ in favorable conditions to non-favorable conditions?
• What would happen if the containers are different sizes will that influence my results in my project?
• What would happen if someone were to have move my potatoes?
6.HYPOTHESIS
The potato exposed to dirt in favorable conditions (warm, moist, dark) will grow more bacteria (fungi growth in mm) than the dirty potatoes in non-favorable conditions (lemon juice) over a period of 7 days (a week).
7.AIM
The aim of this investigation is to see whether potato fungi prefers to grow on a clean non- touched potato or on a dirty potato or on clean potato non-touched but in lemon juice potato or on a dirty potato in lemon juice.
8.VARIABLES
Dependent:
Bacterial growth (mm)
Independent:
Conditions (dirty or clean potatoes with or without lemon
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• Put the rubber gloves back on so that you can put your clean potatoes in to their container.
• Use the fork to pick up the potatoes but make sure that the side where you cut is facing upward for the clean then do the same with the other two halves but take off the gloves before putting them into their containers.
• Put each half in to a container.
• Get your measuring cups and the lemon juice pour 6 teaspoon of lemon juice into two of the container (C(clean), D(dirty)).
• Once you have poured the lemon juice into the two containers get the clingwrap and cut four pieces of the clingwrap with your scissors (make sure that it’s big enough to cover the opening of the container).
• Pull it tight over the opening of the container and makes sure that it is sealed tightly so that nothing can come inside.
• Record the results in a table
12.PRECORTIONS
• Make sure that you wash the containers well before you use them and dry them off with a clan towel.
• Make sure that you don’t burn yourself when you are using the stove.
• Boil the potatoes well to get rid if any
Experiment 1: Materials: • Alka-Seltzer tablets • Empty and clean water or soda bottles (12 oz to 24 oz) • Balloons • Water • Clock • Stove top Procedure: 1. Pour a sufficient amount of water (about 16 oz) into a small pot and place on the stove at high heat. 2. Watch the clock and after 30 seconds take the water off the heat.
Differential media allows for the differentiation between two similar micro-organisms through how the bacteria may handle certain compounds found in the media or the different reactions that may take place when the bacteria is exposed to the medium (3). Selective media on the other hand allow only certain microbes to grow. This is due to the plate containing a limited amount of nutrients, compounds and chemicals that will deter the growth of certain bacteria (3). Dyes, antimicrobial substances, salts, certain growth inhibitors and, antibiotics are also found on this type of medium (3). The differential and selective media mentioned in this lab are as follows:
I thought that it was pretty interesting that just a potato has some significant medicinal properties. So I decided to give it a try, after I just got home my mother screamed and when I went down
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
Additionally, it was difficult obtaining a piece of rhubarb that was thin and particularly red, therefore the effect could not be best observed in the cells. Part B: Design your own experiment Parts of this practical were taken and slightly altered from the following link http://www.markedbyteachers.com/gcse/science/investigate-the-effect-of-surface-area-on-osmosis-in-potato-tissue.html Aim: To observe the effect different surface area: volume ratios have on osmosis in potato tissue. Hypothesis: If the potato has a larger surface area: volume ratio, the quicker osmosis will take place and the larger the mass will be at the end of the experiment, therefore the difference in mass of the potatoes from the start of the experiment to the end of the experiment will be larger. Additionally, the potato pieces left in a saltwater solution will decrease in mass, whereas the pieces left in water will increase in mass.
For this lab I will be using water and sucrose to demonstrate the rate of osmosis. In this lab I will be exploring how temperature impacts the rate of osmosis by placing pieces of potato of equal size in solutions of different temperatures and observing the change in mass of potato after a given period of time. The change in mass will indicate the rate of osmosis.
Independent Variable amount of substrate (sucrose) present 3. Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. I believe glucose and fructose was used as an indicator because they are what produces sucrose and sucrose creates more sucrase activity.
We zeroed out the scale and weighed all four potato cores at once and recorded the mass. We then put those potato cores into the beaker of 75 mL of solution. With the potato cores in the beaker we then put a watch glass over the top of the beaker to minimize the amount of solution that evaporates. We let the potato cores sit in the solution overnight. The next day we then emptied the beaker of the solution by carefully draining the solution, while not letting the potato cores fall out.
When you hear the word bread do you think about food or music? Well of course people will probably say food and look at you strangely. That means they have forgotten or haven’t heard about the rock group which came from Los Angeles, California and was called Bread. Between 1970 and 1977 they had 13 songs which hit the Billboard Hot 100 chart and their kind of music was known as soft rock.
Panera Bread Company What is Panera Bread? They serve quality food with speedy service but not too fast like McDonalds or other fast food restaurants nor as expensive and slow as full dine in restaurants (i.e. Chili’s or Applebee’s). “Panera Bread offers freshly baked artisan bread to neighborhoods in cities throughout the country. As of September 27th, 2016, Panera Bread has 2,024 baker-cafes in 46 states” (panerabread.com).
By using the same mass of potato slices and putting them in different concentration of solutions for a specific amount of time will tell us how the concentration changes the mass of the potato slice. Therefore changing the rate of osmosis. Hypothesis: I predict that, if the piece of potato was put into a solution that has a high concretion of sucrose then the potato slice would lose mass as it would lose water from its cells because the water is moving out of the cell from a high concentration to a low concentration of water through a semi- permeable membrane. The cell is hypotonic and the solution is hypertonic.
Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to
Does bacteria on hands impact with mould growth Introduction: Mold is a part of the fungi family, it grows on almost anything with yeast on it if you leave it out for to long. Mold is often occurs on a yeast product like bread and this happens when the bread is in a warm moist conditions for a period of time like 4-10 days. mold on bread is common because bread provides a desirable source of nutrients for mold. These include the bacteria on the bread and the bread needs to moist and relatively warm and humid for it to take place.
The experiment shall use several concentrations of sucrose solution and a substance known as Methylene blue. A piece of potato/ carrot shall be placed in a boiling tube and the solution shall be poured into it. This tube shall have Methylene blue added into it. After incubation some of this solution shall be taken out with a pipette and inserted into a separate boiling tube containing the same sucrose solution however this solution shall be known as the pre-incubated solution. The drop shall be watched so as to see if the density of the water and concentration of sucrose has increased or not, displaying the water
The percentage weight change is dependent on surface area of potato Controlled The amount of salt solution used Percentage of salt in solution Length of time the potato is sat in water Materials needed 3 Potatoes 600 ml 15% salt solution Utensils 4x 300 ml beaker Knife Cutting board Scale Ruler Procedure 1. 8 rectangular prisms will be cut from the potatoes;