Leptadenia Pyrotech Lab Report

Antibacterial activity of root and stem extracts of Leptadenia pyrotechnica on Klebsiella pneumoniae and Enterobacter aerogenes

Munesh Kumar, Minakshi Lalit, Rajesh Thakur*

Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana – 125001. India.

* Corresponding Author: [e-mail]

Abstract

The in vitro antibacterial activity of Leptadenia pyrotechnica root and stem extracts was studied against Klebsiella pneumoniae and Enterobacter aerogenes by using agar-well diffusion method. Methanol was used as solvent for preparation of extracts. Both plant parts effectively inhibited the growth of both the pathogens; however, more activity was observed against Klebsiella pneumoniae. The activity of both extracts was found to be dose dependent and maximum activity was observed at concentration 60mg/ml for both root and stem extracts.

Keywords

Leptadenia pyrotechnica, Medicinal plants, Well diffusion method, Zone of inhibition

Introduction

The spread of drug resistant bacteria is one of the most serious threats to successful treatment of bacterial diseases. Throughout the world, antibiotics are commonly used against all microbial infections; however,

pneumoniae and E. aerogenes are both gram negative bacteria associated with many health related problems worldwide. K. pneumoniae is a common hospital-acquired pathogen, causing nosocomial pneumonia, urinary tract infections, and intra-abdominal infections29,30. E. aerogenes has emerged as an important hospital pathogen since 199231,32. It is the third leading cause of respiratory tract infections by gram-negative bacteria, after Staphylococcus aureus and Pseudomonas aeruginosa33. Antibiotics are commonly used against these bacteria but the major concern is the increasing antibiotics resistance in these bacteria. Both K. pneumoniae and E. aerogenes show resistance to many broad spectrum antibiotics like carbapenems, cephalosporins and