Part C: Change the amount of the substrate First, the blank was prepared according to table 2 without the enzyme addition. The enzyme was added later after the blank was measured by the spectrophotometer. Table 2: The amount of Sodium Phosphate Buffer pH 7.0, L-Dopa, and enzyme needed in each cuvette.
Accuracy: Reliability and accuracy of the methods were studied based on recovery studies. Analysis was carried in three replicates with the spiking standard ascorbic acid (99.9% purity) concentration of 0.02 mg/ml at the levels of 50%, 100%, and 150%. The % recovery was calculated.
The motivation of this investigation was to achieve 85% of methanol recovery from the distillate. II. Methodology: The distillation column was analyzed theoretically using McCabe Thiele to establish the number of stages required for separation. The vapor-liquid equilibrium (VLE) data for methanol and 2-propanol was used to plot curves of methanol-vapor fraction versus methanol-liquid fraction, and methanol liquid-vapor fraction versus temperature.
In this week’s lab we had to determine the density of a quarter, penny, and dime. My question was “How does is each coin?” Density is the amount of mass in an object. To find the density of each coin in this lab, we used a triple beam balance to find each coin’s mass and a graduated cylinder to find their volumes. With all this information, I can now form a hypothesis.
Using the equation of the titration in the experiment (Equation 1), calculate the number of moles of the ascorbic acid reacting. 4. Then proceed to calculating the concentration in mol. dm3 of the ascorbic acid in the solutions that was obtained. Data Collection and Processing Raw Data Table 1: Amount of Iodine Reacting (± 0.05cm3) in the titration FT 1 FT 2 FT 3 FT 4 FT 5 Trial 1 1.9 1.8 1.8 1.5 1.6 Trial 2 2.1 1.8 1.7 1.5 1.6 Trial 3 2.1
The effect of the solution concentration of sodium chloride on diffusion in yam cores compared to the solution concentration of water Abstract The purpose of the experiment was to see if different solution concentrations had an effect on diffusion. Our group established a hypothesis that stated; sodium chloride will make the yam cores weigh less than in water. In order to start experimenting, we obtained 10 yam cores, weighed them and placed them in five cups that contained 50 mL of water.
The topic of research is, “how fast does an Alka-Seltzer tablet make gas?”. In the experiment, the scientists will be measuring the chemical reaction rates that occur, when 1 Alka-Seltzer tablet is placed in a specific temperature of water. The independent variable during the experiment will be the temperature of the water (degrees Celsius). The dependent variable during the experiment will be, the rate in which gas is produced (in seconds). The constants of the experiment, will be the amount of water used and the Alka Selter compound.
What happens during this stage of the experiment showing visual change and acidic reaction that is becoming weaker as the molecules are deprotonated to become equal parts within the solution. So by finding the molar ratios we learned that acid to base are 1:1 when equally balance or concentrated. So therefore concentrations at stoichiometric end point can be found by plugging in the values to formulate a dilution equation as seen above. When expressing calculations I found when doing the Titration of an Unknown Acid I discovered that with .1 mL of Sodium Hydroxide and at least 10 mL Anthocyanin as/or acetic solution will produce an average molarity of 0.9 or higher according to my
Purpose and Techniques: This experiment has the aim to determine a chemical formula of hydrated compound, which ingrains cupper, chloride and water molecules in its structure. In order to find this hydrated compound, it is necessary to use the law of multiple proportions. In other word, finding the appropriate variables values to this compound (CxCly*zH2O). Additionally, two major steps are required to proceed the experiment.
Serum GOT activity was measured colorimetrically. Non-haemolytic serum was incubated with a buffered mixture of L- aspartate and α- oxoglutarate at 37oC for 12.6 minutes. The initial absorbance was recorded 1 minute after addition of the serum sample and at 1 minute interval thereafter for 3 minutes. The mean absorbance per minute (Δ A/minute) was recorded and used for the calculation of enzyme activity.
Identification of Unknown Solutions and Ammonium Salts preAice Chemistry Lab Report Descriptions of unknown solutions: Unknown Descriptions A Clear, colorless, odorless liquid. B Clear, colorless, odorless liquid. C Clear, red-brown, odorless liquid D Clear, yellow-orange, odorless liquid.