Part C: Change the amount of the substrate First, the blank was prepared according to table 2 without the enzyme addition. The enzyme was added later after the blank was measured by the spectrophotometer. Table 2: The amount of Sodium Phosphate Buffer pH 7.0, L-Dopa, and enzyme needed in each cuvette. Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5 Sodium Phosphate Buffer PH 7.0 (mL) 2.40 2.20 1.80 1.60 1.10 L- Dopa (mL) 0.20 0.40 0.80 1.00 1.50 Enzyme (mL) 0.40 0.40 0.40 0.40 0.40 For example, to prepare the cuvette 1, 2.40 mL of buffer pH 7.0 was measured by the micropipette P-1000, and was added into cuvette labeled #1 for the second set of cuvette. Next, 0.20 mL of L-dopa was measured by the micropipette P-1000 and was added into
Analysis was carried in three replicates with the spiking standard ascorbic acid (99.9% purity) concentration of 0.02 mg/ml at the levels of 50%, 100%, and 150%. The % recovery was calculated. Range: The data generated in precision and linearity was considered for establishment of Range. The sample weight of 1250 mg to 3750 mg was taken for analysis and it was found to be within the linearity and precise. Robustness: Reliability of the method was done about deliberate variations in method parameters like a.
Methodology: The distillation column was analyzed theoretically using McCabe Thiele to establish the number of stages required for separation. The vapor-liquid equilibrium (VLE) data for methanol and 2-propanol was used to plot curves of methanol-vapor fraction versus methanol-liquid fraction, and methanol liquid-vapor fraction versus temperature. III. Results: From the results, the average efficiency was 0.4308, 0.3778, and 0.4956 at 0.57kW supplied (30.58 mL/min Feed; 26.30 mL/min Boil Up), 0.94kW (30.58 mL/min Feed; 52.96 mL/min Boil Up), and 1.27kW (30.58 mL/min Feed; 77.50 mL/min Boil Up) respectively. From the experiment, it was observed that the quality
In this week’s lab we had to determine the density of a quarter, penny, and dime. My question was “How does is each coin?” Density is the amount of mass in an object. To find the density of each coin in this lab, we used a triple beam balance to find each coin’s mass and a graduated cylinder to find their volumes. With all this information, I can now form a hypothesis. My first hypothesis is if I put a quarter in a graduated cylinder and get the volume and weigh it, then it will have the greatest density.
Using the equation of the titration in the experiment (Equation 1), calculate the number of moles of the ascorbic acid reacting. 4. Then proceed to calculating the concentration in mol. dm3 of the ascorbic acid in the solutions that was obtained. Data Collection and Processing Raw Data Table 1: Amount of Iodine Reacting (± 0.05cm3) in the titration FT 1 FT 2 FT 3 FT 4 FT 5 Trial 1 1.9 1.8 1.8 1.5 1.6 Trial 2 2.1 1.8 1.7 1.5 1.6 Trial 3 2.1 2.0 1.8 1.5 1.5 Trial 4 2.5 2.3 1.7 1.6 1.5 Trial 5 1.9 1.8 1.6 1.6 1.4 Average 2.1 1.94 1.72 1.54 1.52 Analysis The results of Table 1 are shown graphically in Figure 2 where the calculated and measured Iodine Solution are plotted with respect to the amount of days.
The effect of the solution concentration of sodium chloride on diffusion in yam cores compared to the solution concentration of water Abstract The purpose of the experiment was to see if different solution concentrations had an effect on diffusion. Our group established a hypothesis that stated; sodium chloride will make the yam cores weigh less than in water. In order to start experimenting, we obtained 10 yam cores, weighed them and placed them in five cups that contained 50 mL of water. At 10-minute intervals, we would take them out and weigh them again for 30 minutes. We repeated this experiment using another five set of cups that contained 50 mL of 1M sodium chloride.
The topic of research is, “how fast does an Alka-Seltzer tablet make gas?”. In the experiment, the scientists will be measuring the chemical reaction rates that occur, when 1 Alka-Seltzer tablet is placed in a specific temperature of water. The independent variable during the experiment will be the temperature of the water (degrees Celsius). The dependent variable during the experiment will be, the rate in which gas is produced (in seconds). The constants of the experiment, will be the amount of water used and the Alka Selter compound.
What happens during this stage of the experiment showing visual change and acidic reaction that is becoming weaker as the molecules are deprotonated to become equal parts within the solution. So by finding the molar ratios we learned that acid to base are 1:1 when equally balance or concentrated. So therefore concentrations at stoichiometric end point can be found by plugging in the values to formulate a dilution equation as seen above. When expressing calculations I found when doing the Titration of an Unknown Acid I discovered that with .1 mL of Sodium Hydroxide and at least 10 mL Anthocyanin as/or acetic solution will produce an average molarity of 0.9 or higher according to my
Purpose and Techniques: This experiment has the aim to determine a chemical formula of hydrated compound, which ingrains cupper, chloride and water molecules in its structure. In order to find this hydrated compound, it is necessary to use the law of multiple proportions. In other word, finding the appropriate variables values to this compound (CxCly*zH2O). Additionally, two major steps are required to proceed the experiment. The first consists to heat a sample to liberate the water hydration, and then compare two mass weights before and after heating so gets easier to find the water percentage (mass).
Serum GOT activity was measured colorimetrically. Non-haemolytic serum was incubated with a buffered mixture of L- aspartate and α- oxoglutarate at 37oC for 12.6 minutes. The initial absorbance was recorded 1 minute after addition of the serum sample and at 1 minute interval thereafter for 3 minutes. The mean absorbance per minute (Δ A/minute) was recorded and used for the calculation of enzyme activity. The absorbance was read at U.V.