Moisture determination is an important indicator of the fertilizer 's quality. Nutrient content is dependent on the amount of moisture it contains. It is necessary to grind the sample so that fineness desired for the experiment is achieved. In this experiment, gravimetric method of analysis was used. Gravimetric analysis is a method that involves precipitating and weighing the mass to determine how much of a certain substance is present.
This experiment aims to separate the components of the green colored food dye and get the TLC profile of each eluent collected. III. Experimental Procedure Before starting with the column chromatography for food dye, the right solvent must be chosen between 2-butanol with acetic acid, ammonia in butanol, 1 part 1-butanol 1 part acetic acid, and 2 parts methanol 1 part water. In choosing the appropriate solvent for column chromatography, the solvent system must give a TLC profile wherein most of the spots are well separated and has a Rf value within 0.3-0.5. For TLC profiling, 4 TLC plates were prepared for the testing of each solvent.
Water acts as a leaving group in the third step and is removed from the reaction intermediate. In the fourth step, the molecule undergoes deprotonation with the help of the concentrated sulfuric acid to form isopentyl acetate. The reaction that was carried out in the experiment was a reversible reaction. In order to obtain as much isopentyl acetate as possible, Le Chatelier’s principle was used to ensure that we were able to collect a sufficient amount of isopentyl acetate. Le Chatelier’s principle says that if you disturb a system in equilibrium the equilibrium will shift in order to account for the disturbance.
First generation biofuel is made from food crops or feed stocks that can also be consumed as human food by extracting the oils through fermentation. Crops like wheat, sugarcane, corn, sugar beet and sorghum are some of the main sources of producing biofuel. Since their availability and extraction through conventional means they are termed as conventional biofuels. Most common first generation biofuel includes:- (1) Biodiesel. Extracted from vegetable oils, animal fats, seeds from plants like Soybeans, Canola, and Sunflower are few of the examples.
The first method is broth culture. In broth culture, Escherichia coli are grown in a liquid medium. It is shown that the nutrient broth appeared to have sediment. This shows that Escherichia coli carries out anaerobic and aerobic respiration as it can survive anywhere in the broth. The second method is agar slope culture where Escherichia coli are grown on a slant agar in a test tube.
PHYTOCHEMICALS Legumes contain a number of phytochemicals that have potential health benefits as well as some that can reduce the bioavailability of nutrients. These compounds include saponins, phytic acid, phenolic compounds, enzyme inhibitors, and lectins. Saponins Saponins are amphiphilic compounds present in a wide variety of plants and herbs. Structurally, saponins in food exist as glycosides, with a hydrophobic triterpenoid or steroid (sapogenin) group linked to water-soluble sugar residues. Saponins have been shown to have anticarcinogenic and antimutagenic properties in a variety of in vitro approaches (cell culture) .
These micelles are imperative for their polar negativity, which causes a pull towards the positive pole. The molecules that are hydrophobic (water hating) will tend to aggregate with the micelle, while those that are hydrophilic (water loving) will move fairly quickly through the solution. The key parameters for this technique are pH, surfactant concentration, any additives and the polymer coatings that are used on the capillary wall. Capillary zone electrophoresis (CZE) This is the most commonly used capillary electrophoresis method of the six being discussed. CZE, also known as free solution capillary electrophoresis, is a separation technique that predominantly takes into account the ratio of the particle’s charge to mass, where those with large charge to mass ratio separate from the rest first; therefore, the larger the ratio, the quicker the separation.
actic acid bacteria: one of the most important group of microorganism its associated with plants, meats , and dairy. lactic acid produced from carbohydrates and sugars. its also consider a strong fertilizer and harmful microorganism. one of lactic acid benefits are empowering the productions of important sources of energy in your body which are glucose and glycogen in the liver. so therefore it helps gain energy.
Also, in their study, they were able to determine the ration of nitrogen present on the plant by Kjeldahl method, this is also used to estimate the protein. The carbohydrate, organic acids and sterols were determined by using HPLC. Also, it was stated in the study that Rhubarb has minerals: this includes Vitamin A and C, thiamin, niacin, potassium and phosphorus. The percentage of protein on rhubarb is 2.861% while nitrogen has 16.311% present on the plant. The carbohydrates present on the plant: Fructose has a concentration of 55.71, Glucose has a concentration of 54.17, and Sucrose has a concentration of 18.76.