Lyophyllum Shimeji Case Study

Source of culture strain

The strain of Lyophyllum shimeji Chinese strain will be obtained from the culture collection Center for Tropical Mushroom Research and Development (CTMRD), Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Munoz, Nueva Ecija.

Preparation of Culture Media Eight grams of powdered potato dextrose agar (PDA) will be accurately weighed and boiled in 200 mL of distilled water with constant stirring until dissolved and become homogenous. The solution will be dispensed in a clean flat bottle. The bottle will be plugged with cotton and covered with clean paper and sterilized using an autoclave at 121°C or 15 pounds per inch (psi) for 20 minutes. Approximately,

Mycelia of Lyophyllum shimeji will be allowed to grow on the medium at room temperature.

SUB-STUDY 1. Growth Performance of Secondary Mycelia of L. shimeji in Different Indigenous Culture Media

The mycelial growth performance will be evaluated using different indigenous culture media namely; Rice bran decoction sucrose gulaman (RBDSG); Yellow corn grit decoction sucrose gulaman (YCGDSG); Potato sucrose decoction gulaman (PSG); and Coconut water decoction gulaman (CWG). Each locally available indigenous culture media were served as treatment of the study. The best indigenous culture media will be recorded for fastest short incubation period and mycelial density. Every indigenous culture media will serve as treatments of the study, and each treatment will be replicated trice. The most appropriate indigenous culture media will be determined by shortest incubation period of total mycelial ramification, mycelial density and daily mycelial growth.

Preparation, Sterilization and Pour Plating of Culture

After boiling, the spawning materials will be air dried until 65% moisture will be reached. 40 g of each spawn material will be dispensed in a clean glass bottle. The bottled spawning materials will be plugged with cotton, wrapped with paper and sterilized by autoclaving at 15 psi or 121°C for 45 minutes. After sterilization and cooling, substrate will be inoculated with 8 mm mycelial discs from the pure culture of L. shimeji. Inoculated spawn will be incubated under optimum temperature condition to allow full mycelial