Maladies Lab Report

MDA level was determined by thiobarbituric acid reactive substances (TBARS) in serum, based on the reaction between MDA and TBARS. Standard Malondialdehyde solution in 5 mL of volume was processed along with test samples. 1.5 mL of 0.8% of TBA was added to 1 mL of the serum sample. Then 0.4 mL of 8.1% sodium dodecyl sulphate and 1.5 mL of acetic acid was added. The mixture was finally made upto 5 mL with distilled water and placed in hot water bath at 95ºC for 1 h. After cooling, 1 mL of distilled water and 5 mL of the mixture of n-butanol and pyridine (15:1, v/v) was added.

Lastly, a glucose fermentation test was conducted, P. aeruginosa was aseptically transferred into a tube of glucose broth using an inoculating loop. The tube was then placed into an incubator at 37 degrees Celsius for 48 hours. Once the time had elapsed the tube appeared pink which indicated a negative test. With the negative result the unknown species was identified as P.

From the Unknown tube professor Cooper gave me, I scratched a little on the slant surface with the sterilized inoculating loop. Then I place it on a clean prepared slide which already had a slight drop of water. The two substances are mixed together in the middle of the slide and let dry completely. One extra step of “heat fix” is necessary to adhere everything to the surface of the slide. To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water.

Four drops of acetone was then placed inside and the test tube was then shaken thoroughly. The procedure was then repeated to the formaldehyde, cyclohexanone, and benzaldehyde. To the acetone and formaldehyde test tubes, 3 drops of 6M sodium hydroxide was placed since no discoloration was observed after shaking the test tube. It was observed that the acetone turned purple to green to yellow to brown yellow after the sodium hydroxide was mixed with it while the formaldehyde turned yellow brown after the sodium hydroxide was added which indicated a positive result. The cyclohexanone produce red-orange precipitate while the benzaldehyde produced yellow

The absorbance was set to 0 Abs while the spectrometer was set to ʎmax (from Part A). In Part B, 1.00 ml of the solution was mixed with the Blue dye in the beaker and half-way covered with a cuvette. Concurrently, the Spectronic 20 was blanked with water. The processes detailed above were repeated, each at a time.

N-(1-Carboxymethyl-1H-tetrazol-5-yl)-hydrazinium nitrate (3). A solution of AgNO3 (0.10 g, 0.60 mmol) in distilled water (1.5 mL) was added dropwise in the dark to the solution of compound 2 (0.10 g, 0.60 mmol) in Deionized water (1.5 mL) under stirring. After 2-3 hour, the precipitate was filtered, and rinsed with 4 mL distilled water. The solvent was removed by rotary Evaporation to produce a white solid at 88% yield (0.10 g); N-(1-Carboxymethyl-1H-tetrazol-5-yl)-hydrazinium nitrate: Yield: 88%; yellow crystals;. IR (KBr): 3396, 3329, 3140, 3008, 1628, 1494, 1383 cm-1; UV (H2O): λmax = 293-296

3MHNO3, 1ml of Agno3 and unknown compound formed precipitate. Conclusion After doing flame test, solubility test and PH test, the groups able to identify the unknown compound, after all of the tests and reactions were performed, it was found that the unknown compound was chlorine, it is solubility in the water. The flame test matches the color of calcium chorine indicating that the unknown compound contained chorine. We also used ice to see whether unknown compound will dissolve in the ice.

There are various clinician-administered measures for the assessment of OCD. Clinical interviews and various self-report measures are typically used as well as behavioral avoidance tests or observational tasks in which individuals are exposed to feared stimuli while rating their distress level. It is also important to review the etiological considerations when assessing and diagnosing OCD. Being that OCD involves both genetic and environmental factors. Heredity plays a major role in the etiology of OCD, therefore it would be beneficial to examine if any of Lady Macbeth’s family members have OCD or related disorders.

In the study conducted among health care workers in Iraq on Knowledge, attitude and practices regarding TB, it was found that even though health staff had relatively higher knowledge with respect to what to do at any given time, it did not translate into their practice of TB control especially in the area of investigation of a TB suspect, a situation that fostered healthy grounds for poor case finding and inappropriate case-holding practices (Hashim et al., 2005). Again, the study on knowledge attitude and practices of healthcare workers regarding TB control among thirty government hospitals in Thailand also arrived at the conclusion that high healthcare providers' knowledge does not necessarily translate into good practice. Knowledge level was found to be in correlation with the number of TB trainings attended as well as the profession of the respondent (Lertkanokkun and Okanurak, 2013). 2.3 TB case detection Practices among healthcare

Then, putting of choloform in amount of 10 mL and solution of Hanus iodine as amount of 10 mL into conical flask is realized. Addition of these two substance into otheraflask also occurs for blank. Next, waiting for these two samples for exactly 30 minutes is realized. Afterthat, solution of potassium iodine in amount of 15 mL and 40 mL water being distilled are added. Titration of last mixture is performed in company with 0.1 M Na2S2O3 until the obtaining of color in yellow.

Next, we obtained an Erlenmeyer flask for the titration. Rinse it with DI water. Next we are to dispense from the buret at least mL of the designated acid into the flask. Record the initial and final volume readings on the buret in laboratory notebook data table. Next we add 20mL of deionized water and anthocyanin indicator drop

All the absorbances were remained 0 for the blank. After 120 seconds, the blank was then removed, and the appropriate amount of enzyme Tyrosinase (0.40 mL) was measured and added into the blank (cuvette #1) using the micropipette P-1000 according to the table 2. The final volume in the cuvette was 3mL. The cuvette contained the enzyme sample was wiped off with a KimWipe and was placed into the sample compartment of the machine. The sample compartment door was closed.

Another five tea bags were soaked for fifteen seconds in beaker B and then removed. The same teabags were then placed into beaker C for two minutes. They were removed after the time elapsed. 4. The solutions were allowed to cool to room temperature using an ice bath.

The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.