Mangifera Indica Lab Report

Materials and Methods

Preparation of test extracts

Fresh plants of the mistletoe growing on Mangifera indica were collected during flowering in the month of August, 2009 from Kasaragod District of Kerala. Voucher specimen of the plant (no. 00637) was deposited at the Pharmacognosy department of Captain Srinivasa Murti Drug Research Institute for Ayurveda, Chennai. Shade dried whole plant material was extracted with ethanol and double distilled water by cold percolation. The aqueous extract (HEAq) was concentrated and dried in freeze drier to obtain dark brown gummy material and the alcoholic extract (HEAl) was concentrated over a water bath to obtain dark brown gummy material.

Animals

The animals were obtained from the animal facility attached to the pharmacology laboratory of Central Research Institute for Siddha. The experiments were carried out after obtaining permission from Institutional animal ethics committee (Approval number 79/PHARMA/SCRI/2010). Animals were exposed to

The mice were fasted overnight and provided with water ad libitum and divided into 4 groups of three animals each. The alcohol and the aqueous extracts were suspended in 1% sodium carboxyl methyl cellulose (SCMC)5. The starting dose level of aqueous and alcoholic extracts of H. elastica was 2000 mg/kg body weight p.o. as most of the crude extracts possess LD50 value more than 2000 mg/kg. After oral administration, the mice were observed on an hourly basis for 24 h to assess the general behavior, any signs of toxicity, and mortality. They were further observed for 14 days for toxic symptoms and mortality. Mice were weighed before starting and ending acute toxicity and any changes in skin fur, eyes, mucous membranes, central nervous system and behavioral pattern were observed. Tremors, convulsions, salivation, diarrhea, lethargy, sleep, coma and any specific severe sign were also